Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 5 September 1986:
Vol. 233. no. 4768, pp. 1073 - 1076
DOI: 10.1126/science.3738525
Prev | Table of Contents | Next

Articles

Science, Vol 233, Issue 4768, 1073-1076
Copyright © 1986 by American Association for the Advancement of Science

articles

Transition state analogs as ligands for affinity purification of juvenile hormone esterase

YA Abdel-Aal and BD Hammock

Insect juvenile hormones are metabolized in numerous species of caterpillars by low abundance, highly specific esterases. Because of their role in regulating and possibly disrupting juvenile hormone titer and thus insect metamorphosis, they are of interest to developmental biologists as well as scientists interested in selective insect control. However, the enzymes have defied attempts to purify and characterize them. Juvenile hormone esterase activity can be inhibited by a variety of 3-substituted 1,1,1-trifluoropropanone sulfides. These apparent transition state analogs were used as ligands and eluting agents to purify juvenile hormone esterase from four insect species from 500-fold to over 1000-fold in high yield. After elution from the affinity column, the enzymes were radiolabeled with paraoxon and analyzed by electrophoresis, and the results demonstrate a high degree of purity. Transition state analogs may be useful for the affinity purification of other enzymes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase.
A. Tan, H. Tanaka, T. Tamura, and T. Shiotsuki (2005)
PNAS 102, 11751-11756
   Abstract »    Full Text »    PDF »
A Novel Protein That Binds Juvenile Hormone Esterase in Fat Body Tissue and Pericardial Cells of the Tobacco Hornworm Manduca sexta L..
M. Shanmugavelu, A. R. Baytan, J. D. Chesnut, and B. C. Bonning (2000)
J. Biol. Chem. 275, 1802-1806
   Abstract »    Full Text »    PDF »
Chemistry of pheromone and hormone metabolism in insects.
G. Prestwich (1987)
Science 237, 999-1006
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)