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Science 5 August 1988:
Vol. 241. no. 4866, pp. 703 - 705
DOI: 10.1126/science.2456617
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Articles

Science, Vol 241, Issue 4866, 703-705
Copyright © 1988 by American Association for the Advancement of Science

articles

An E. coli promoter that regulates transcription by DNA superhelix-induced cruciform extrusion

MS Horwitz and LA Loeb

Joseph Gottstein Memorial Cancer Research Laboratory, Department of Pathology, University of Washington, Seattle 98195.

DNA can form structures other than the Watson-Crick double helix. The potential contributions to gene regulation from one such structure have been investigated by assembling a promoter capable of adopting cruciform base-pairing. Transcription from this promoter by RNA polymerase in vitro was repressed as the cruciform was extruded by increasing negative DNA supercoiling. Transcription in vivo was induced as supercoiling was relaxed by growth in conditions that inhibit DNA gyrase. A DNA conformational change is therefore capable of regulating the initiation of transcription.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Transcriptional Repression by Binding of Poly(ADP-ribose) Polymerase to Promoter Sequences.
V. A. Soldatenkov, S. Chasovskikh, V. N. Potaman, I. Trofimova, M. E. Smulson, and A. Dritschilo (2002)
J. Biol. Chem. 277, 665-670
   Abstract »    Full Text »
PBF-2 Is a Novel Single-Stranded DNA Binding Factor Implicated in PR-10a Gene Activation in Potato.
D. Desveaux, C. Després, A. Joyeux, R. Subramaniam, and N. Brisson (2000)
PLANT CELL 12, 1477-1490
   Abstract »    Full Text »
Cloning and Nucleotide Sequence of the DNA Gyrase gyrA Gene from Serratia marcescens and Characterization of Mutations in gyrA of Quinolone-Resistant Clinical Isolates.
J. H. Kim, E. H. Cho, K. S. Kim, H. Y. Kim, and Y. M. Kim (1998)
Antimicrob. Agents Chemother. 42, 190-193
   Abstract »    Full Text »
Supercoil-induced extrusion of a regulatory DNA hairpin.
X. Dai, M. B. Greizerstein, K. Nadas-Chinni, and L. B. Rothman-Denes (1997)
PNAS 94, 2174-2179
   Abstract »    Full Text »    PDF »
Targeted Melting and Binding of a DNA Regulatory Element by a Transactivator of c-myc.
L. Bazar, D. Meighen, V. Harris, R. Duncan, D. Levens, and M. Avigan (1995)
J. Biol. Chem. 270, 8241-8248
   Abstract »    Full Text »    PDF »
Specific recognition of cruciform DNA by nuclear protein HMG1.
M. Bianchi, M Beltrame, and G Paonessa (1989)
Science 243, 1056-1059
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)