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Science 12 August 1988:
Vol. 241. no. 4867, pp. 823 - 825
DOI: 10.1126/science.3406740
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Articles

Science, Vol 241, Issue 4867, 823-825
Copyright © 1988 by American Association for the Advancement of Science

articles

Relaxation of isolated ventricular cardiomyocytes by a voltage-dependent process

JH Bridge, KW Spitzer, and PR Ershler

Nora Eccles Harrison Cardiovascular Research and Training Institute, Salt Lake City, UT.

Cell contraction and relaxation were measured in single voltage-clamped guinea pig cardiomyocytes to investigate the contribution of sarcolemmal Na+-Ca2+ exchange to mechanical relaxation. Cells clamped from -80 to 0 millivolts displayed initial phasic and subsequent tonic contractions; caffeine reduced or abolished the phasic and enlarged the tonic contraction. The rate of relaxation from tonic contractions was steeply voltage-dependent and was significantly slowed in the absence of a sarcolemmal Na+ gradient. Tonic contractions elicited in the absence of a Na+ gradient promptly relaxed when external Na+ was applied, reflecting activation of Na+-Ca2+ exchange. It appears that a voltage-dependent Na+-Ca2+ exchange can rapidly mechanically relax mammalian heart muscle.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)