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Science 19 August 1988:
Vol. 241. no. 4868, pp. 965 - 967
DOI: 10.1126/science.3043665

Articles

Science, Vol 241, Issue 4868, 965-967
Copyright © 1988 by American Association for the Advancement of Science


articles

Mammalian glucocorticoid receptor derivatives enhance transcription in yeast

M Schena and KR Yamamoto

Department of Biochemistry and Biophysics, University of California, San Francisco 94143.

In mammalian cells, the glucocorticoid receptor binds specifically to glucocorticoid response element (GRE) DNA sequences and enhances transcription from linked promoters. It is shown here that derivatives of the glucocorticoid receptor also enhance transcription when expressed in yeast. Receptor-mediated enhancement in yeast was observed in fusions of GRE sequences to the yeast cytochrome c1 (CYC1) promoter; the CYC1 upstream activator sequences were not essential, since enhancement was observed in fusions of GREs to mutant CYC1 promoters retaining only the TATA region and transcription startpoints. It is concluded that the receptor operates by a common, highly conserved mechanism in yeast and mammalian cells.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)