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Science 2 September 1988:
Vol. 241. no. 4870, pp. 1188 - 1191
DOI: 10.1126/science.3413482
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Articles
Science, Vol 241, Issue 4870, 1188-1191
Copyright © 1988 by American Association for the Advancement of Science
Induction of an antibody that catalyzes the hydrolysis of an amide bond
KD Janda,
D Schloeder,
SJ Benkovic,
and
RA Lerner
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.
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