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Science 9 September 1988:
Vol. 241. no. 4871, pp. 1335 - 1338
DOI: 10.1126/science.3413495

Articles

Science, Vol 241, Issue 4871, 1335-1338
Copyright © 1988 by American Association for the Advancement of Science


articles

Physical analysis of transcription preinitiation complex assembly on a class II gene promoter

MW Van Dyke, RG Roeder, and M Sawadogo

Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021.

Transcription of protein-encoding genes by human RNA polymerase II requires multiple ancillary proteins (transcription factors). Interactions between these proteins and the promoter DNA of a viral class II gene (the major late transcription unit of adenovirus) were investigated by enzymatic and chemical footprinting. The experiments indicated that the assembly of functionally active RNA polymerase II-containing transcription preinitiation complexes requires a complete set of transcription factors, and that both specific protein-DNA and protein-protein interactions are involved. This allows individual steps along the transcription reaction pathway to be tested directly, thus providing a basis for understanding basic transcription initiation mechanisms as well as the regulatory processes that act on them.


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Recycling of the general transcription factors during RNA polymerase II transcription..
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Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation..
K Yokomori, M P Zeidler, J L Chen, C P Verrijzer, M Mlodzik, and R Tjian (1994)
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Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism..
D T Auble, K E Hansen, C G Mueller, W S Lane, J Thorner, and S Hahn (1994)
Genes & Dev. 8, 1920-1934
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TFIID sequence recognition of the initiator and sequences farther downstream in Drosophila class II genes..
B A Purnell, P A Emanuel, and D S Gilmour (1994)
Genes & Dev. 8, 830-842
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Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex..
K Yokomori, A Admon, J A Goodrich, J L Chen, and R Tjian (1993)
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TBP, a universal eukaryotic transcription factor?.
N Hernandez (1993)
Genes & Dev. 7, 1291-1308
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An ATP-dependent inhibitor of TBP binding to DNA..
D T Auble and S Hahn (1993)
Genes & Dev. 7, 844-856
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DNA repair and transcription: the helicase connection.
S Buratowski (1993)
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Synergism in transcriptional activation: a kinetic view..
D Herschlag and F B Johnson (1993)
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The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence..
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Differential regulation of transcription preinitiation complex assembly by activator and repressor homeo domain proteins..
F B Johnson and M A Krasnow (1992)
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Promoter melting and TFIID complexes on Drosophila genes in vivo..
C Giardina, M Perez-Riba, and J T Lis (1992)
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Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter..
Q Zhou, P M Lieberman, T G Boyer, and A J Berk (1992)
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The acidic activator GAL4-AH can stimulate polymerase II transcription by promoting assembly of a closed complex requiring TFIID and TFIIA..
W Wang, J D Gralla, and M Carey (1992)
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SPT3 interacts with TFIID to allow normal transcription in Saccharomyces cerevisiae..
D M Eisenmann, K M Arndt, S L Ricupero, J W Rooney, and F Winston (1992)
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DNA binding provides a signal for phosphorylation of the RNA polymerase II heptapeptide repeats..
S R Peterson, A Dvir, C W Anderson, and W S Dynan (1992)
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Transcription factor IID mutants defective for interaction with transcription factor IIA.
S Buratowski and H Zhou (1992)
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TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters..
J Colgan and J L Manley (1992)
Genes & Dev. 6, 304-315
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Polymerase II promoter activation: closed complex formation and ATP-driven start site opening.
W Wang, M Carey, and J. Gralla (1992)
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Coactivators for a proline-rich activator purified from the multisubunit human TFIID complex..
N Tanese, B F Pugh, and R Tjian (1991)
Genes & Dev. 5, 2212-2224
   Abstract »    PDF »
RNA polymerase II carboxy-terminal domain contributes to the response to multiple acidic activators in vitro..
S M Liao, I C Taylor, R E Kingston, and R A Young (1991)
Genes & Dev. 5, 2431-2440
   Abstract »    PDF »
The Zta trans-activator protein stabilizes TFIID association with promoter DNA by direct protein-protein interaction..
P M Lieberman and A J Berk (1991)
Genes & Dev. 5, 2441-2454
   Abstract »    PDF »
The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro..
S M Lobo, J Lister, M L Sullivan, and N Hernandez (1991)
Genes & Dev. 5, 1477-1489
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Repression of HIV-1 transcription by a cellular protein.
H Kato, M Horikoshi, and R. Roeder (1991)
Science 251, 1476-1479
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A plant DNA-binding protein increases the number of active preinitiation complexes in a human in vitro transcription system..
F Katagiri, K Yamazaki, M Horikoshi, R G Roeder, and N H Chua (1990)
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A TATA-like sequence located downstream of the transcription initiation site is required for expression of an RNA polymerase II transcribed gene..
J Carcamo, E Maldonado, P Cortes, M H Ahn, I Ha, Y Kasai, J Flint, and D Reinberg (1990)
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Cloning of the Schizosaccharomyces pombe TFIID gene reveals a strong conservation of functional domains present in Saccharomyces cerevisiae TFIID..
A Hoffmann, M Horikoshi, C K Wang, S Schroeder, P A Weil, and R G Roeder (1990)
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Functional domains and upstream activation properties of cloned human TATA binding protein.
M. Peterson, N Tanese, B. Pugh, and R Tjian (1990)
Science 248, 1625-1630
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Cloning of a transcriptionally active human TATA binding factor.
C. Kao, P. Lieberman, M. Schmidt, Q Zhou, R Pei, and A. Berk (1990)
Science 248, 1646-1650
   Abstract »    PDF »
Sarkosyl defines three intermediate steps in transcription initiation by RNA polymerase III: application to stimulation of transcription by E1A..
R Kovelman and R G Roeder (1990)
Genes & Dev. 4, 646-658
   Abstract »    PDF »
DNA regions that regulate the ovarian transcriptional specificity of Drosophila yolk protein genes..
S K Logan, M J Garabedian, and P C Wensink (1989)
Genes & Dev. 3, 1453-1461
   Abstract »    PDF »
The role of cis-acting promoter elements in tissue-specific albumin gene expression.
P Maire, J Wuarin, and U Schibler (1989)
Science 244, 343-346
   Abstract »    PDF »
The coactivator dTAFII110/hTAFII135 is sufficient to recruit a polymerase complex and activate basal transcription mediated by CREB.
E. A. Felinski and P. G. Quinn (2001)
PNAS 98, 13078-13083
   Abstract »    Full Text »    PDF »



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Science. ISSN 0036-8075 (print), 1095-9203 (online)