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Science 16 September 1988:
Vol. 241. no. 4872, pp. 1503 - 1506
DOI: 10.1126/science.3420405
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Articles

Science, Vol 241, Issue 4872, 1503-1506
Copyright © 1988 by American Association for the Advancement of Science

articles

Hormone-sensitive lipase: sequence, expression, and chromosomal localization to 19 cent-q13.3

C Holm, TG Kirchgessner, KL Svenson, G Fredrikson, S Nilsson, CG Miller, JE Shively, C Heinzmann, RS Sparkes, T Mohandas, and al. et

Department of Medical and Physiological Chemistry, University of Lund, Sweden.

Hormone-sensitive lipase, a key enzyme in fatty acid mobilization, overall energy homeostasis, and possibly steroidogenesis, is acutely controlled through reversible phosphorylation by catecholamines and insulin. The 757-amino acid sequence predicted from a cloned rat adipocyte complementary DNA showed no homology with any other known lipase or protein. The activity-controlling phosphorylation site was localized to Ser563 in a markedly hydrophilic domain, and a lipid-binding consensus site was tentatively identified. One or several messenger RNA species (3.3, 3.5, or 3.9 kilobases) were expressed in adipose and steroidogenic tissues and heart and skeletal muscle. The human hormone-sensitive lipase gene mapped to chromosome 19 cent-q13.3.


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