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Science 7 October 1988:
Vol. 242. no. 4875, pp. 67 - 69
DOI: 10.1126/science.2845575
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Articles

Science, Vol 242, Issue 4875, 67-69
Copyright © 1988 by American Association for the Advancement of Science

articles

Regulation of a heart potassium channel by protein kinase A and C

KB Walsh and RS Kass

Department of Physiology, University of Rochester, School of Medicine and Dentistry, NY 14642.

The enzymes adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase (protein kinase A) and protein kinase C regulate the activity of a diverse group of cellular proteins including membrane ion channel proteins. When protein kinase A was stimulated in cardiac ventricular myocytes with the membrane-soluble cAMP analog 8-chlorphenylthio cAMP (8-CPT cAMP), the amplitude of the delayed-rectifier potassium current (IK) doubled when recorded at 32 degrees C but was not affected at 22 degrees C. In contrast, modulation of the calcium current (ICa) by 8-CPT cAMP was independent of temperature with similar increases in ICa occurring at both temperatures. Stimulation of protein kinase C by phorbol 12,13-dibutyrate also enhanced IK in a temperature-dependent manner but failed to increase ICa at either temperature. Thus, cardiac delayed-rectifier potassium but not calcium channels are regulated by two distinct protein kinases in a similar temperature-dependent fashion.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)