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Science 21 October 1988:
Vol. 242. no. 4877, pp. 419 - 423
DOI: 10.1126/science.242.4877.419
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Articles

Herbicide Resistance in Transgenic Plants Expressing a Bacterial Detoxification Gene

DAVID M. STALKER , KEVIN E. MCBRIDE , and LORRAINE D. MALYJ

The herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) is a photosynthetic (photosystem II) inhibitor in plants. A gene, bxn, encoding a specific nitrilase that converts bromoxynil to its primary metabolite 3,5-dibromo-4-hydroxybenzoic acid, was cloned from the natural soil bacterium Klebsiella ozaenae. For expression in plants, the bxn gene was placed under control of a light-regulated tissue-specific promoter, the ribulose bisphosphate carboxylase small subunit. Transfer of this chimeric gene and expression of a bromoxynil-specific nitrilase in leaves of transgenic tobacco plants conferred resistance to high levels of a commercial formulation of bromoxynil. The results presented indicate a successful approach to obtain herbicide resistance by introducing a novel catabolic detoxification gene in plants.

Submitted on April 29, 1988
Accepted on August 9, 1988


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Science. ISSN 0036-8075 (print), 1095-9203 (online)