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Science 21 October 1988:
Vol. 242. no. 4877, pp. 423 - 426
DOI: 10.1126/science.3140379
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Articles

Science, Vol 242, Issue 4877, 423-426
Copyright © 1988 by American Association for the Advancement of Science

articles

Single-chain antigen-binding proteins

RE Bird, KD Hardman, JW Jacobson, S Johnson, BM Kaufman, SM Lee, T Lee, SH Pope, GS Riordan, and M Whitlow

Genex Corporation, Gaithersburg, MD 20877.

Single-chain antigen-binding proteins are novel recombinant polypeptides, composed of an antibody variable light-chain amino acid sequence (VL) tethered to a variable heavy-chain sequence (VH) by a designed peptide that links the carboxyl terminus of the VL sequence to the amino terminus of the VH sequence. These proteins have the same specificities and affinities for their antigens as the monoclonal antibodies whose VL and VH sequences were used to construct the recombinant genes that were expressed in Escherichia coli. Three of these proteins, one derived from the sequence for a monoclonal antibody to growth hormone and two derived from the sequences of two different monoclonal antibodies to fluorescein, were designed, constructed, synthesized, purified, and assayed. These proteins are expected to have significant advantages over monoclonal antibodies in a number of applications.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)