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Science 28 October 1988:
Vol. 242. no. 4878, pp. 564 - 567
DOI: 10.1126/science.3175673
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Articles

Science, Vol 242, Issue 4878, 564-567
Copyright © 1988 by American Association for the Advancement of Science

articles

Xeroderma pigmentosum group E cells lack a nuclear factor that binds to damaged DNA

G Chu and E Chang

Department of Medicine, Stanford University School of Medicine, CA 94305.

The disease xeroderma pigmentosum is characterized by deficient repair of damaged DNA. Fusions of cells from different patients have defined nine genetic complementation groups (A through I), implying that DNA repair in humans involves multiple gene products. In this report, an extension of the gel electrophoresis binding assay was used to identify at least one nuclear factor that (i) bound to DNA damaged by ultraviolet radiation or the antitumor drug cisplatin, but (ii) was notably absent in cells from complementation group E. Therefore, the factor appears to participate in a versatile DNA repair pathway at the stage of binding and recognition.


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The DDB1-CUL4ADDB2 ubiquitin ligase is deficient in xeroderma pigmentosum group E and targets histone H2A at UV-damaged DNA sites.
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Sequential binding of UV DNA damage binding factor and degradation of the p48 subunit as early events after UV irradiation.
V. Rapic-Otrin, M. P. McLenigan, D. C. Bisi, M. Gonzalez, and A. S. Levine (2002)
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T. Tan and G. Chu (2002)
Mol. Cell. Biol. 22, 3247-3254
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UV-damaged DNA-binding Proteins Are Targets of CUL-4A-mediated Ubiquitination and Degradation.
X. Chen, Y. Zhang, L. Douglas, and P. Zhou (2001)
J. Biol. Chem. 276, 48175-48182
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The Xeroderma Pigmentosum Group E Gene Product DDB2 Is a Specific Target of Cullin 4A in Mammalian Cells.
A. Nag, T. Bondar, S. Shiv, and P. Raychaudhuri (2001)
Mol. Cell. Biol. 21, 6738-6747
   Abstract »    Full Text »    PDF »
DNA Damage in the Nucleosome Core Is Refractory to Repair by Human Excision Nuclease.
R. Hara, J. Mo, and A. Sancar (2000)
Mol. Cell. Biol. 20, 9173-9181
   Abstract »    Full Text »
The Paramyxovirus Simian Virus 5 V Protein Slows Progression of the Cell Cycle.
G. Y. Lin and R. A. Lamb (2000)
J. Virol. 74, 9152-9166
   Abstract »    Full Text »
DNA Damage Recognition and Nucleotide Excision Repair in Mammalian Cells.
R.D. WOOD, S.J. ARAUJO, R.R. ARIZA, D.P. BATTY, M. BIGGERSTAFF, E. EVANS, P.-H. GAILLARD, D. GUNZ, B. KOBERLE, I. KURAOKA, et al. (2000)
Cold Spring Harb Symp Quant Biol 65, 173-182
   Abstract »    PDF »
Cullin 4A Associates with the UV-damaged DNA-binding Protein DDB.
P. Shiyanov, A. Nag, and P. Raychaudhuri (1999)
J. Biol. Chem. 274, 35309-35312
   Abstract »    Full Text »    PDF »
Characterization of DNA Recognition by the Human UV-damaged DNA-binding Protein.
Y. Fujiwara, C. Masutani, T. Mizukoshi, J. Kondo, F. Hanaoka, and S. Iwai (1999)
J. Biol. Chem. 274, 20027-20033
   Abstract »    Full Text »    PDF »
The Naturally Occurring Mutants of DDB Are Impaired in Stimulating Nuclear Import of the p125 Subunit and E2F1-Activated Transcription.
P. Shiyanov, S. A. Hayes, M. Donepudi, A. F. Nichols, S. Linn, B. L. Slagle, and P. Raychaudhuri (1999)
Mol. Cell. Biol. 19, 4935-4943
   Abstract »    Full Text »    PDF »
Molecular mechanism of nucleotide excision repair.
W. L. de Laat, N. G.J. Jaspers, and J. H.J. Hoeijmakers (1999)
Genes & Dev. 13, 768-785
   Full Text »
Expression of the p48 xeroderma pigmentosum gene is p53-dependent and is involved in global genomic repair.
B. J. Hwang, J. M. Ford, P. C. Hanawalt, and G. Chu (1999)
PNAS 96, 424-428
   Abstract »    Full Text »    PDF »
p48 Activates a UV-Damaged-DNA Binding Factor and Is Defective in Xeroderma Pigmentosum Group E Cells That Lack Binding Activity.
B. J. Hwang, S. Toering, U. Francke, and G. Chu (1998)
Mol. Cell. Biol. 18, 4391-4399
   Abstract »    Full Text »
Proteins that specifically recognize cisplatin-damaged DNA: a clue to anticancer activity of cisplatin.
J. Zlatanova, J. Yaneva, and S. H. Leuba (1998)
FASEB J 12, 791-799
   Abstract »    Full Text »
Relationship of the Xeroderma Pigmentosum Group E DNA Repair Defect to the Chromatin and DNA Binding Proteins UV-DDB and Replication Protein A.
V. Otrin, I. Kuraoka, T. Nardo, M. McLenigan, A. P. M. Eker, M. Stefanini, A. S. Levine, and R. D. Wood (1998)
Mol. Cell. Biol. 18, 3182-3190
   Abstract »    Full Text »
The DNA-dependent ATPase Activity of Yeast Nucleotide Excision Repair Factor 4 and Its Role in DNA Damage Recognition.
S. N. Guzder, P. Sung, L. Prakash, and S. Prakash (1998)
J. Biol. Chem. 273, 6292-6296
   Abstract »    Full Text »    PDF »
DDB, a Putative DNA Repair Protein, Can Function as a Transcriptional Partner of E2F1.
S. Hayes, P. Shiyanov, X. Chen, and P. Raychaudhuri (1998)
Mol. Cell. Biol. 18, 240-249
   Abstract »    Full Text »
Translocation of a UV-damaged DNA binding protein into a tight association with chromatin after treatment of mammalian cells with UV light.
V. Otrin, M McLenigan, M Takao, A. Levine, and M Protic (1997)
J. Cell Sci. 110, 1159-1168
   Abstract »    PDF »
Mutations Specific to the Xeroderma Pigmentosum Group E Ddb- Phenotype.
A. F. Nichols, P. Ong, and S. Linn (1996)
J. Biol. Chem. 271, 24317-24320
   Abstract »    Full Text »    PDF »
Excision Repair in Man and the Molecular Basis of Xeroderma Pigmentosum Syndrome.
J.T. Reardon, L.H. Thompson, and A. Sancar (1993)
Cold Spring Harb Symp Quant Biol 58, 605-617
   Abstract »    PDF »
Nucleotide Excision Repair of DNA by Mammalian Cell Extracts and Purified Proteins.
R.D. Wood, A. Aboussekhra, M. Biggerstaff, C.J. Jones, A. O'donovan, M.K.K. Shivji, and D.E. Szymkowski (1993)
Cold Spring Harb Symp Quant Biol 58, 625-632
   Abstract »    PDF »
Specific binding of chromosomal protein HMG1 to DNA damaged by the anticancer drug cisplatin.
P. Pil and S. Lippard (1992)
Science 256, 234-237
   Abstract »    PDF »
DDB Accumulates at DNA Damage Sites Immediately after UV Irradiation and Directly Stimulates Nucleotide Excision Repair.
M. Wakasugi, A. Kawashima, H. Morioka, S. Linn, A. Sancar, T. Mori, O. Nikaido, and T. Matsunaga (2002)
J. Biol. Chem. 277, 1637-1640
   Abstract »    Full Text »    PDF »
Human Damage-specific DNA-binding Protein p48. CHARACTERIZATION OF XPE MUTATIONS AND REGULATION FOLLOWING UV IRRADIATION.
A. F. Nichols, T. Itoh, J. A. Graham, W. Liu, M. Yamaizumi, and S. Linn (2000)
J. Biol. Chem. 275, 21422-21428
   Abstract »    Full Text »    PDF »
Nuclear Transport of Human DDB Protein Induced by Ultraviolet Light.
W. Liu, A. F. Nichols, J. A. Graham, R. Dualan, A. Abbas, and S. Linn (2000)
J. Biol. Chem. 275, 21429-21434
   Abstract »    Full Text »    PDF »
Damaged DNA-binding Protein DDB Stimulates the Excision of Cyclobutane Pyrimidine Dimers in Vitro in Concert with XPA and Replication Protein A.
M. Wakasugi, M. Shimizu, H. Morioka, S. Linn, O. Nikaido, and T. Matsunaga (2001)
J. Biol. Chem. 276, 15434-15440
   Abstract »    Full Text »    PDF »


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