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Science 28 October 1988:
Vol. 242. no. 4878, pp. 571 - 574
DOI: 10.1126/science.2845582
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Articles

Science, Vol 242, Issue 4878, 571-574
Copyright © 1988 by American Association for the Advancement of Science

articles

T cell CD3-zeta eta heterodimer expression and coupling to phosphoinositide hydrolysis

M Mercep, JS Bonifacino, P Garcia-Morales, LE Samelson, RD Klausner, and JD Ashwell

Biological Response Modifiers Program, National Cancer Institute, Bethesda, MD 20892.

The T cell antigen receptor consists of an antigen-binding heterodimer that is noncovalently associated with at least five CD3 subunits (gamma, delta, epsilon, zeta, and eta). The CD3-zeta chains are either disulfide-linked homodimers (CD3-zeta 2) or disulfide-linked heterodimers with eta (CD3-zeta eta). Variants of a murine antigen-specific T cell hybridoma that express normal amounts of CD3-zeta 2 but decreased amounts of CD3-zeta eta were isolated. When activated, the parental cell line increased both phosphatidylinositol hydrolysis and serine-specific protein kinase activity to a much greater extent than the variants. In contrast, the activation of a tyrosine-specific kinase after stimulation with a cross-linking antibody to CD3 was similar among these cells. There was a positive linear relation between the expression of CD3-zeta eta and phosphoinositide hydrolysis stimulated by the TCR, suggesting a differential coupling of the T cell alpha beta heterodimer to signal transduction mechanisms due to alpha beta association with either CD3-zeta 2 or CD3-zeta eta.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Regulation of Constitutive TCR Internalization by the {zeta}-Chain.
U. D'Oro, I. Munitic, G. Chacko, T. Karpova, J. McNally, and J. D. Ashwell (2002)
J. Immunol. 169, 6269-6278
   Abstract »    Full Text »    PDF »
Activation of polyphosphoinositide hydrolysis in T cells by H-2 alloantigen but not MLS determinants.
A. O'Rourke, M. Mescher, and S. Webb (1990)
Science 249, 171-174
   Abstract »    PDF »
Structural mutations of the T cell receptor zeta chain and its role in T cell activation.
S. Frank, B. Niklinska, D. Orloff, M Mercep, J. Ashwell, and R. Klausner (1990)
Science 249, 174-177
   Abstract »    PDF »
A cell culture model for T lymphocyte clonal anergy.
R. Schwartz (1990)
Science 248, 1349-1356
   Abstract »    PDF »
T cell antigen receptor-mediated activation of phospholipase C requires tyrosine phosphorylation.
T Mustelin, K. Coggeshall, N Isakov, and A Altman (1990)
Science 247, 1584-1587
   Abstract »    PDF »
Activation-driven programmed cell death and T cell receptor zeta eta expression.
M Mercep, A. Weissman, S. Frank, R. Klausner, and J. Ashwell (1989)
Science 246, 1162-1165
   Abstract »    PDF »


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