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Science 4 November 1988:
Vol. 242. no. 4879, pp. 773 - 777
DOI: 10.1126/science.2847315
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Articles

Science, Vol 242, Issue 4879, 773-777
Copyright © 1988 by American Association for the Advancement of Science

articles

Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice

DW Tank, M Sugimori, JA Connor, and RR Llinas

Molecular Biophysics Research Department, AT&T Bell Laboratories, Murray Hill, NJ 07974.

Microfluorometric imaging was used to study the correlation of intracellular calcium concentration with voltage-dependent electrical activity in guinea pig cerebellar Purkinje cells. The spatiotemporal dynamics of intracellular calcium concentration are demonstrated during spontaneous and evoked activity. The results are in agreement with hypotheses of dendritic segregation of calcium conductances suggested by electrophysiological experiments. These in vitro slice fluorescence imaging methods are applicable to a wide range of problems in central nervous system biochemical and electrophysiological functions.


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