Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 18 November 1988:
Vol. 242. no. 4881, pp. 1036 - 1037
DOI: 10.1126/science.3143155
Prev | Table of Contents | Next

Articles

Science, Vol 242, Issue 4881, 1036-1037
Copyright © 1988 by American Association for the Advancement of Science

articles

Induction of kappa transcription by interferon-gamma without activation of NF-kappa B

M Briskin, MD Kuwabara, DS Sigman, and R Wall

Molecular Biology Institute, UCLA School of Medicine, University of California 90024.

The induction of immunoglobulin kappa light chain expression in 70Z/3 pre-B cells treated with bacterial lipopolysaccharide (LPS) requires the activation of the B cell-specific factor NF-kappa B, which binds to the kappa enhancer motif, GGGACTTTCC. This sequence alone can function as a tissue-specific enhancer for LPS-induced gene expression. A potent inhibitor of B lymphopoiesis [transforming growth factor-beta (TGF-beta)] was used to explore the mechanisms in the activation of kappa transcription by LPS and by interferon-gamma (IFN-gamma). TGF-beta inhibited LPS-induced kappa transcription but not the activation and in vitro binding of NF-kappa B. This indicates that NF-kappa B activation, while necessary, is not sufficient for LPS-induced kappa transcription. TGF-beta had no effect on IFN-gamma-induced kappa transcription, and NF-kappa B was not activated by IFN-gamma. These results reveal that LPS and IFN-gamma activate transcription through different mechanisms.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The I{kappa}B Kinase Complex and NF-{kappa}B Act as Master Regulators of Lipopolysaccharide-Induced Gene Expression and Control Subordinate Activation of AP-1.
D. Krappmann, E. Wegener, Y. Sunami, M. Esen, A. Thiel, B. Mordmuller, and C. Scheidereit (2004)
Mol. Cell. Biol. 24, 6488-6500
   Abstract »    Full Text »    PDF »
Soluble CD14 enriched in colostrum and milk induces B cell growth and differentiation.
D. Filipp, K. Alizadeh-Khiavi, C. Richardson, A. Palma, N. Paredes, O. Takeuchi, S. Akira, and M. Julius (2001)
PNAS 98, 603-608
   Abstract »    Full Text »    PDF »
Transcriptional Regulation of the Ig{kappa} Gene by Promoter-Proximal Pausing of RNA Polymerase II.
E. E. Raschke, T. Albert, and D. Eick (1999)
J. Immunol. 163, 4375-4382
   Abstract »    Full Text »    PDF »
Induction of Early B Cell Factor (EBF) and Multiple B Lineage Genes by the Basic Helix-Loop-Helix Transcription Factor E12.
B. L. Kee and C. Murre (1998)
J. Exp. Med. 188, 699-713
   Abstract »    Full Text »    PDF »
Pentoxifylline Inhibits Ig {kappa} Gene Transcription and Rearrangements in Pre-B Cells.
W. Wang, S. Rath, J. M. Durdik, and R. Sen (1998)
J. Immunol. 160, 1789-1795
   Abstract »    Full Text »    PDF »
The Interferon-inducible Protein Kinase PKR Modulates the Transcriptional Activation of Immunoglobulin kappa Gene.
A. E. Koromilas, C. Cantin, A. W. B. Craig, R. Jagus, J. Hiscott, and N. Sonenberg (1995)
J. Biol. Chem. 270, 25426-25434
   Abstract »    Full Text »    PDF »
Activating Transcription Factor 1 and Cyclic AMP Response Element Modulator Can Modulate the Activity of the Immunoglobulin [IMAGE] 3` Enhancer.
J. M. R. Pongubala and M. L. Atchison (1995)
J. Biol. Chem. 270, 10304-10313
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)