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Science 18 November 1988:
Vol. 242. no. 4881, pp. 1040 - 1042
DOI: 10.1126/science.3194753

Articles

Science, Vol 242, Issue 4881, 1040-1042
Copyright © 1988 by American Association for the Advancement of Science


articles

Multiple principal sigma factor homologs in eubacteria: identification of the "rpoD box"

K Tanaka, T Shiina, and H Takahashi

Institute of Applied Microbiology, University of Tokyo, Japan.

Genes for the principal sigma factor (rpoD genes) of various eubacteria were identified with a synthetic oligonucleotide probe corresponding to a conserved sequence in rpoD gene products of Escherichia coli and Bacillus subtilis. Multiple rpoD homologs were found in the strains of Micrococcus, Pseudomonas, and Streptomyces, whereas single genes were detected in E. coli, B. subtilis, and Staphylococcus aureus. The four rpoD homologs of Streptomyces coelicolor A3(2) were cloned and sequenced. A homologous portion with 13 amino acids was found in the rpoD genes of S. coelicolor A3(2), E. coli, and B. subtilis and was named the "rpoD box."


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Positive Regulation of Sugar Catabolic Pathways in the Cyanobacterium Synechocystis sp. PCC 6803 by the Group 2 {sigma} Factor SigE.
T. Osanai, Y. Kanesaki, T. Nakano, H. Takahashi, M. Asayama, M. Shirai, M. Kanehisa, I. Suzuki, N. Murata, and K. Tanaka (2005)
J. Biol. Chem. 280, 30653-30659
   Abstract »    Full Text »    PDF »
Characterization of the Primary sigma Factor of Staphylococcus aureus.
R. Deora and T. K. Misra (1996)
J. Biol. Chem. 271, 21828-21834
   Abstract »    Full Text »    PDF »



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