Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 18 November 1988:
Vol. 242. no. 4881, pp. 1050 - 1053
DOI: 10.1126/science.2461586
Prev | Table of Contents | Next

Articles

Science, Vol 242, Issue 4881, 1050-1053
Copyright © 1988 by American Association for the Advancement of Science

articles

Identification of synaptophysin as a hexameric channel protein of the synaptic vesicle membrane

L Thomas, K Hartung, D Langosch, H Rehm, E Bamberg, WW Franke, and H Betz

Zentrum fur Molekulare Biologie, Universitat Heidelberg, Federal Republic of Germany.

The quaternary structure and functional properties of synaptophysin, a major integral membrane protein of small presynaptic vesicles, were investigated. Cross-linking and sedimentation studies indicate that synaptophysin is a hexameric homo-oligomer, which in electron micrographs exhibits structural features common to channel-forming proteins. On reconstitution into planar lipid bilayers, purified synaptophysin displays voltage-sensitive channel activity with an average conductance of about 150 picosiemens. Because specific channels and fusion pores have been implicated in vesicular uptake and release of secretory compounds, synaptophysin may have a role in these processes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Synaptic tetraspan vesicle membrane proteins are conserved but not needed for synaptogenesis and neuronal function in Caenorhabditis elegans.
C. Abraham, H. Hutter, M. T. Palfreyman, G. Spatkowski, R. M. Weimer, R. Windoffer, E. M. Jorgensen, and R. E. Leube (2006)
PNAS 103, 8227-8232
   Abstract »    Full Text »    PDF »
Control of neurotransmitter release by an internal gel matrix in synaptic vesicles.
D. Reigada, I. Diez-Perez, P. Gorostiza, A. Verdaguer, I. Gomez de Aranda, O. Pineda, J. Vilarrasa, J. Marsal, J. Blasi, J. Aleu, et al. (2003)
PNAS 100, 3485-3490
   Abstract »    Full Text »    PDF »
The Synaptic Vesicle Protein Synaptophysin: Purification and Characterization of Its Channel Activity.
D. Gincel and V. Shoshan-Barmatz (2002)
Biophys. J. 83, 3223-3229
   Abstract »    Full Text »    PDF »
Fluorescence Resonance Energy Transfer Detection of Synaptophysin I and Vesicle-associated Membrane Protein 2 Interactions during Exocytosis from Single Live Synapses.
M. Pennuto, D. Dunlap, A. Contestabile, F. Benfenati, and F. Valtorta (2002)
Mol. Biol. Cell 13, 2706-2717
   Abstract »    Full Text »    PDF »
Pantophysin Is a Phosphoprotein Component of Adipocyte Transport Vesicles and Associates with GLUT4-containing Vesicles.
C. C. Brooks, P. E. Scherer, K. Cleveland, J. L. Whittemore, H. F. Lodish, and B. Cheatham (2000)
J. Biol. Chem. 275, 2029-2036
   Abstract »    Full Text »    PDF »
Abnormal Features in Skeletal Muscle from Mice Lacking Mitsugumin29.
M. Nishi, S. Komazaki, N. Kurebayashi, Y. Ogawa, T. Noda, M. Iino, and H. Takeshima (1999)
J. Cell Biol. 147, 1473-1480
   Abstract »    Full Text »    PDF »
Ion Channels in Presynaptic Nerve Terminals and Control of Transmitter Release.
A. Meir, S. Ginsburg, A. Butkevich, S. G. Kachalsky, I. Kaiserman, R. Ahdut, S. Demirgoren, and R. Rahamimoff (1999)
Physiol Rev 79, 1019-1088
   Abstract »    Full Text »    PDF »
Functional Importance of Synaptobrevin and SNAP-25 during Exocytosis of Histamine by Rat Gastric Enterochromaffin-Like Cells.
B. Hohne-Zell, A. Galler, W. Schepp, M. Gratzl, and C. Prinz (1997)
Endocrinology 138, 5518-5526
   Abstract »    Full Text »    PDF »
Isoform-specific, Calcium-regulated Interaction of the Synaptic Vesicle Proteins SV2 and Synaptotagmin.
A. E. Schivell, R. H. Batchelor, and S. M. Bajjalieh (1996)
J. Biol. Chem. 271, 27770-27775
   Abstract »    Full Text »    PDF »
The V(0) Sector of the V-ATPase, Synaptobrevin, and Synaptophysin Are Associated on Synaptic Vesicles in a Triton X-100-resistant, Freeze-thawing Sensitive, Complex.
T. Galli, P. S. McPherson, and P. De Camilli (1996)
J. Biol. Chem. 271, 2193-2198
   Abstract »    Full Text »    PDF »
Inhibition of Neurotransmitter Release by Synthetic Proline-rich Peptides Shows That the N-terminal Domain of Vesicle-associated Membrane Protein/Synaptobrevin Is Critical for Neuro-exocytosis.
F. Cornille, F. Deloye, M.-C. Fournié-Zaluski, B. P. Roques, and B. Poulain (1995)
J. Biol. Chem. 270, 16826-16832
   Abstract »    Full Text »    PDF »
The Biochemistry of Neurotransmitter Secretion.
S. M. Bajjalieh and R. H. Scheller (1995)
J. Biol. Chem. 270, 1971-1974
   Full Text »    PDF »
The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains.
R. Leube (1995)
J. Cell Sci. 108, 883-894
   Abstract »    PDF »
Synaptic vesicle phosphoproteins and regulation of synaptic function.
P Greengard, F Valtorta, A. Czernik, and F Benfenati (1993)
Science 259, 780-785
   Abstract »    PDF »
The mechanics of biological membrane fusion. Merger of aspects from electron microscopy and patch-clamp analysis.
H. Plattner, G. Knoll, and C. Erxleben (1992)
J. Cell Sci. 103, 613-618
   PDF »
Calcium-dependent transmitter secretion reconstituted in Xenopus oocytes: requirement for synaptophysin.
J Alder, B Lu, F Valtorta, P Greengard, and M. Poo (1992)
Science 257, 657-661
   Abstract »    PDF »
Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones.
M. Bennett, N Calakos, and R. Scheller (1992)
Science 257, 255-259
   Abstract »    PDF »
Molecular Analysis of Proteins Associated with the Synaptic Vesicle Membrane.
J.K. Ngsee, W.S. Trimble, L.A. Elferink, B. Wendland, K. Miller, N. Calakos, and R.H. Scheller (1990)
Cold Spring Harb Symp Quant Biol 55, 111-118
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)