Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 18 November 1988:
Vol. 242. no. 4881, pp. 1053 - 1056
DOI: 10.1126/science.3194757
Prev | Table of Contents | Next

Articles

Science, Vol 242, Issue 4881, 1053-1056
Copyright © 1988 by American Association for the Advancement of Science

articles

The presence of fibroblast growth factor in the frog egg: its role as a natural mesoderm inducer

D Kimelman, JA Abraham, T Haaparanta, TM Palisi, and MW Kirschner

Department of Biochemistry and Biophysics, University of California, San Francisco 94143.

A complementary DNA clone corresponding to a 4.2-kilobase transcript that is present in the Xenopus oocyte and newly transcribed in the neurula stages of development has been isolated. This messenger RNA encodes a 155-amino acid protein that is 84% identical to the human basic fibroblast growth factor (bFGF). When expressed in Escherichia coli and purified, the Xenopus FGF induced mesoderm in animal cell blastomeres as measured by muscle actin expression. Immunoblots with an antibody to a Xenopus FGF peptide show that the oocyte and early embryo contain a store of the FGF polypeptide at high enough concentrations to induce mesoderm. The presence of FGF in the oocyte, together with the apparent lack of a secretory signal sequence in the protein, suggest that the regulation of mesoderm induction may involve novel mechanisms that occur after the translation of FGF.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Fibroblast Growth Factor Signaling during Early Vertebrate Development.
R. T. Bottcher and C. Niehrs (2005)
Endocr. Rev. 26, 63-77
   Abstract »    Full Text »    PDF »
Enhancement of Fibronectin Fibrillogenesis and Bone Formation by Basic Fibroblast Growth Factor via Protein Kinase C-Dependent Pathway in Rat Osteoblasts.
C.-H. Tang, R.-S. Yang, T.-H. Huang, S.-H. Liu, and W.-M. Fu (2004)
Mol. Pharmacol. 66, 440-449
   Abstract »    Full Text »    PDF »
RNA editing and regulation of Drosophila 4f-rnp expression by sas-10 antisense readthrough mRNA transcripts.
N. T. PETERS, J. A. ROHRBACH, B. A. ZALEWSKI, C. M. BYRKETT, and J. C. VAUGHN (2003)
RNA 9, 698-710
   Abstract »    Full Text »    PDF »
eFGF and its mode of action in the community effect during Xenopus myogenesis.
H. Standley, A. Zorn, and J. Gurdon (2001)
Development 128, 1347-1357
   Abstract »    PDF »
The VT+ and VT- Isoforms of the Fibroblast Growth Factor Receptor Type 1 Are Differentially Expressed in the Presumptive Mesoderm of Xenopus Embryos and Differ in Their Ability to Mediate Mesoderm Formation.
G. D. Paterno, P. J. Ryan, K. R. Kao, and L. L. Gillespie (2000)
J. Biol. Chem. 275, 9581-9586
   Abstract »    Full Text »    PDF »
Axis determination by inhibition of Wnt signaling in Xenopus.
K. Itoh and S. Y. Sokol (1999)
Genes & Dev. 13, 2328-2336
   Abstract »    Full Text »
FGF-18, a Novel Member of the Fibroblast Growth Factor Family, Stimulates Hepatic and Intestinal Proliferation.
M. C.-T. Hu, W. R. Qiu, Y.-p. Wang, D. Hill, B. D. Ring, S. Scully, B. Bolon, M. DeRose, R. Luethy, W. S. Simonet, et al. (1998)
Mol. Cell. Biol. 18, 6063-6074
   Abstract »    Full Text »
Differential Regulation of Urokinase-type Plasminogen Activator Expression by Basic Fibroblast Growth Factor and Serum in Myogenesis. REQUIREMENT OF A COMMON MITOGEN-ACTIVATED PROTEIN KINASE PATHWAY.
F. Miralles, D. Ron, M. Baiget, J. Felez, and P. Munoz-Canoves (1998)
J. Biol. Chem. 273, 2052-2058
   Abstract »    Full Text »    PDF »
cDNA Cloning of a Novel, Developmentally Regulated Immediate Early Gene Activated by Fibroblast Growth Factor and Encoding a Nuclear Protein.
G. D. Paterno, Y. Li, H. A. Luchman, P. J. Ryan, and L. L. Gillespie (1997)
J. Biol. Chem. 272, 25591-25595
   Abstract »    Full Text »    PDF »
Characterization and tissue-specific expression of the rat basic fibroblast growth factor antisense mRNA and protein.
R. Knee, A. W. Li, and P. R. Murphy (1997)
PNAS 94, 4943-4947
   Abstract »    Full Text »    PDF »
A cDNA Encoding Fish Fibroblast Growth Factor-2, Which Lacks Alternative Translation Initiation.
J.-i. Hata, J. Takeo, C. Segawa, and S. Yamashita (1997)
J. Biol. Chem. 272, 7285-7289
   Abstract »    Full Text »    PDF »
eFGF, Xcad3 and Hox genes form a molecular pathway that establishes the anteroposterior axis in Xenopus.
M. Pownall, A. Tucker, J. Slack, and H. Isaacs (1996)
Development 122, 3881-3892
   Abstract »    PDF »
Cloning of a Fibroblast Growth Factor Receptor 1 Splice Variant from Xenopus Embryos That Lacks a Protein Kinase C Site Important for the Regulation of Receptor Activity.
L. L. Gillespie, G. Chen, and G. D. Paterno (1995)
J. Biol. Chem. 270, 22758-22763
   Abstract »    Full Text »    PDF »
Cell-Cell Signaling in Early Cortical Development.
A. R. Kriegstein (1995)
Neuroscientist 1, 268-276
   Abstract »    PDF »
Fibroblast growth factor is a direct neural inducer, which combined with noggin generates anterior-posterior neural pattern.
T. Lamb and R. Harland (1995)
Development 121, 3627-3636
   Abstract »    PDF »
Properties of the dorsal activity found in the vegetal cortical cytoplasm of Xenopus eggs.
T Holowacz and R. Elinson (1995)
Development 121, 2789-2798
   Abstract »    PDF »
FGF is a prospective competence factor for early activin-type signals in Xenopus mesoderm induction.
R. Cornell, T. Musci, and D Kimelman (1995)
Development 121, 2429-2437
   Abstract »    PDF »
Effect of activin and lithium on isolated Xenopus animal blastomeres and response alteration at the midblastula transition.
K Kinoshita and M Asashima (1995)
Development 121, 1581-1589
   Abstract »    PDF »
Ectoderm induces muscle-specific gene expression in Drosophila embryos.
R Baker and G Schubiger (1995)
Development 121, 1387-1398
   Abstract »    PDF »
Role of MAP kinase in mesoderm induction and axial patterning during Xenopus development.
C LaBonne, B Burke, and M Whitman (1995)
Development 121, 1475-1486
   Abstract »    PDF »
Regulation of Spemann organizer formation by the intracellular kinase Xgsk-3.
S. Pierce and D Kimelman (1995)
Development 121, 755-765
   Abstract »    PDF »
Vertebrate embryonic induction: mesodermal and neural patterning.
D. Kessler and D. Melton (1994)
Science 266, 596-604
   Abstract »    PDF »
Heparan sulfate proteoglycans are required for mesoderm formation in Xenopus embryos.
K. Itoh and S. Y. Sokol (1994)
Development 120, 2703-2711
   Abstract »    PDF »
Disruption of mesoderm and axis formation in fish by ectopic expression of activin variants: the role of maternal activin..
J Wittbrodt and F M Rosa (1994)
Genes & Dev. 8, 1448-1462
   Abstract »    PDF »
Wnt-3a regulates somite and tailbud formation in the mouse embryo..
S Takada, K L Stark, M J Shea, G Vassileva, J A McMahon, and A P McMahon (1994)
Genes & Dev. 8, 174-189
   Abstract »    PDF »
Isolation and developmental expression of the amphibian homolog of the fibroblast growth factor receptor 3.
D. Shi, V Fromentoux, C Launay, M Umbhauer, and J. Boucaut (1994)
J. Cell Sci. 107, 417-425
   Abstract »    PDF »
Distinct developmental expression of a new avian fibroblast growth factor receptor.
C Marcelle, A Eichmann, O Halevy, C Breant, and N. Le Douarin (1994)
Development 120, 683-694
   Abstract »    PDF »
Activin-mediated mesoderm induction requires FGF.
R. Cornell and D Kimelman (1994)
Development 120, 453-462
   Abstract »    PDF »
Mesoderm induction by activin requires FGF-mediated intracellular signals.
C LaBonne and M Whitman (1994)
Development 120, 463-472
   Abstract »    PDF »
Basic fibroblast growth factor induces differentiation of neural tube and neural crest lineages of cultured ectoderm cells from Xenopus gastrula.
M. Kengaku and H. Okamoto (1993)
Development 119, 1067-1078
   Abstract »    PDF »
Induction of the Xenopus organizer: expression and regulation of Xnot, a novel FGF and activin-regulated homeo box gene..
G von Dassow, J E Schmidt, and D Kimelman (1993)
Genes & Dev. 7, 355-366
   Abstract »    PDF »
Loss of cytoplasmic basic fibroblast growth factor from physiologically wounded myofibers of normal and dystrophic muscle.
M. Clarke, R Khakee, and P. McNeil (1993)
J. Cell Sci. 106, 121-133
   Abstract »    PDF »
Distribution of acidic and basic fibroblast growth factors in ovine skin during follicle morphogenesis.
D. du Cros, K Isaacs, and G. Moore (1993)
J. Cell Sci. 105, 667-674
   Abstract »    PDF »
Later embryogenesis: regulatory circuitry in morphogenetic fields.
E. Davidson (1993)
Development 118, 665-690
   Abstract »    PDF »
FGF signalling in the early specification of mesoderm in Xenopus.
E Amaya, P. Stein, T. Musci, and M. Kirschner (1993)
Development 118, 477-487
   Abstract »    PDF »
Two FGF-receptor homologues of Drosophila: one is expressed in mesodermal primordium in early embryos.
E Shishido, S Higashijima, Y Emori, and K Saigo (1993)
Development 117, 751-761
   Abstract »    PDF »
The frog prince-ss: a molecular formula for dorsoventral patterning in Xenopus..
H L Sive (1993)
Genes & Dev. 7, 1-12
   PDF »
Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus..
J L Christian and R T Moon (1993)
Genes & Dev. 7, 13-28
   Abstract »    PDF »
Patterns of cell motility in the organizer and dorsal mesoderm of Xenopus laevis.
J. Shih and R. Keller (1992)
Development 116, 915-930
   Abstract »    PDF »
Isolation of Sna, a mouse gene homologous to the Drosophila genes snail and escargot: its expression pattern suggests multiple roles during postimplantation development.
D. E. Smith, F. Franco del Amo, and T. Gridley (1992)
Development 116, 1033-1039
   Abstract »    PDF »
Spatially restricted expression of fibroblast growth factor receptor-2 during Xenopus development.
R. Friesel and S. A. Brown (1992)
Development 116, 1051-1058
   Abstract »    PDF »
Synergistic principles of development: overlapping patterning systems in Xenopus mesoderm induction.
D. Kimelman, J. L. Christian, and R. T. Moon (1992)
Development 116, 1-9
   Abstract »    PDF »
Lithium-sensitive production of inositol phosphates during amphibian embryonic mesoderm induction.
J. Maslanski, L Leshko, and W. Busa (1992)
Science 256, 243-245
   Abstract »    PDF »
Differential expression and regulation of two distinct fibroblast growth factor receptors during early development of the urodele amphibian Pleurodeles waltl.
D. Shi, J. Feige, J. Riou, D. DeSimone, and J. Boucaut (1992)
Development 116, 261-273
   Abstract »    PDF »
Developmental expression of the Xenopus int-2 (FGF-3) gene: activation by mesodermal and neural induction.
D Tannahill, H. Isaacs, M. Close, G Peters, and J. Slack (1992)
Development 115, 695-702
   Abstract »    PDF »
Mesoderm and Jaw Development in Vertebrates: The Role of Growth Factors.
N. C. Milos (1992)
Critical Reviews in Oral Biology & Medicine 4, 73-91
   Abstract »    Full Text »    PDF »
Pattern formation during animal development.
D. Melton (1991)
Science 252, 234-241
   Abstract »    PDF »
The Drosophila FGF-R homolog is expressed in the embryonic tracheal system and appears to be required for directed tracheal cell extension..
L Glazer and B Z Shilo (1991)
Genes & Dev. 5, 697-705
   Abstract »    PDF »
"Pure" human hematopoietic progenitors: permissive action of basic fibroblast growth factor.
M Gabbianelli, M Sargiacomo, E Pelosi, U Testa, G Isacchi, and C Peschle (1990)
Science 249, 1561-1564
   Abstract »    PDF »
A mouse macrophage factor induces head structures and organizes a body axis in Xenopus.
S Sokol, G. Wong, and D. Melton (1990)
Science 249, 561-564
   Abstract »    PDF »
Purification and complementary DNA cloning of a receptor for basic fibroblast growth factor.
P. Lee, D. Johnson, L. Cousens, V. Fried, and L. Williams (1989)
Science 245, 57-60
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)