Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 9 December 1988:
Vol. 242. no. 4884, pp. 1396 - 1402
DOI: 10.1126/science.2904698
Prev | Table of Contents | Next

Articles

Science, Vol 242, Issue 4884, 1396-1402
Copyright © 1988 by American Association for the Advancement of Science

articles

Living with clathrin: its role in intracellular membrane traffic

FM Brodsky

Department of Pharmacy, School of Pharmacy, University of California, San Francisco 94143.

Clathrin polymerization at the cytoplasmic side of the plasma membrane forms coated pits and vesicles that mediate uptake of cell surface receptors. Clathrin-coated vesicles have also been implicated in protein export but definition of their precise role has been controversial. Recent advances in characterization of the clathrin subunits and additional coated vesicle components have identified molecular interactions involved in clathrin polymerization and coated vesicle formation, and have provided new approaches to investigating its function. These studies suggest that clathrin's role, in both inward and outward membrane traffic, is to facilitate receptor transport by a concentration and sorting process that initiates targeting to specific intracellular compartments.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Plant Defense Elicitor Cryptogein Stimulates Clathrin-Mediated Endocytosis Correlated with Reactive Oxygen Species Production in Bright Yellow-2 Tobacco Cells.
N. Leborgne-Castel, J. Lherminier, C. Der, J. Fromentin, V. Houot, and F. Simon-Plas (2008)
Plant Physiology 146, 1255-1266
   Abstract »    Full Text »    PDF »
Calcyon, a Novel Partner of Clathrin Light Chain, Stimulates Clathrin-mediated Endocytosis.
J. Xiao, R. Dai, L. Negyessy, and C. Bergson (2006)
J. Biol. Chem. 281, 15182-15193
   Abstract »    Full Text »    PDF »
Role of clathrin in the regulated secretory pathway of pancreatic {beta}-cells.
M. Molinete, S. Dupuis, F. M. Brodsky, and P. A. Halban (2002)
J. Cell Sci. 114, 3059-3066
   Abstract »    Full Text »    PDF »
Recruitment of Activated G Protein-coupled Receptors to Pre-existing Clathrin-coated Pits in Living Cells.
M. G. H. Scott, A. Benmerah, O. Muntaner, and S. Marullo (2002)
J. Biol. Chem. 277, 3552-3559
   Abstract »    Full Text »    PDF »
Clathrin plays a novel role in the regulation of cell polarity, pseudopod formation, uropod stability and motility in Dictyostelium.
D Wessels, J Reynolds, O Johnson, E Voss, R Burns, K Daniels, E Garrard, T. O'Halloran, and D. Soll (2000)
J. Cell Sci. 113, 21-36
   Abstract »    PDF »
Processing of CFTR bearing the P574H mutation differs from wild-type and deltaF508-CFTR.
L. Ostedgaard, B Zeiher, and M. Welsh (1999)
J. Cell Sci. 112, 2091-2098
   Abstract »    PDF »
Assembly of the Epithelial Na+ Channel Evaluated Using Sucrose Gradient Sedimentation Analysis.
C. Cheng, L. S. Prince, P. M. Snyder, and M. J. Welsh (1998)
J. Biol. Chem. 273, 22693-22700
   Abstract »    Full Text »    PDF »
Endocytic Clathrin-coated Pit Formation Is Independent of Receptor Internalization Signal Levels.
F. Santini, M. S. Marks, and J. H. Keen (1998)
Mol. Biol. Cell 9, 1177-1194
   Abstract »    Full Text »
A Dominant-negative Clathrin Mutant Differentially Affects Trafficking of Molecules with Distinct Sorting Motifs in the Class II Major Histocompatibility Complex (MHC) Pathway.
S.-H. Liu, M. S. Marks, and F. M. Brodsky (1998)
J. Cell Biol. 140, 1023-1037
   Abstract »    Full Text »    PDF »
Partitioning of Proteins into Plasma Membrane Microdomains. CLUSTERING OF MUTANT INFLUENZA VIRUS HEMAGGLUTININS INTO COATED PITS DEPENDS ON THE STRENGTH OF THE INTERNALIZATION SIGNAL.
E. Fire, C. M. Brown, M. G. Roth, Y. I. Henis, and N. O. Petersen (1997)
J. Biol. Chem. 272, 29538-29545
   Abstract »    Full Text »    PDF »
A novel role for clathrin in cytokinesis.
M. L. Niswonger and T. J. O'Halloran (1997)
PNAS 94, 8575-8578
   Abstract »    Full Text »    PDF »
Clathrin heavy chain is required for spore cell but not stalk cell differentiation in Dictyostelium discoideum.
M. Niswonger and T. O'Halloran (1997)
Development 124, 443-451
   Abstract »    PDF »
The Ear of alpha-Adaptin Interacts with the COOH-terminal Domain of the Eps15 Protein.
A. Benmerah, B. Bègue, A. Dautry-Varsat, and N. Cerf-Bensussan (1996)
J. Biol. Chem. 271, 12111-12116
   Abstract »    Full Text »    PDF »
A membrane-associated protein complex with selective binding to the clathrin coat adaptor AP1.
W. Mallet and F. Brodsky (1996)
J. Cell Sci. 109, 3059-3068
   Abstract »    PDF »
Plant clathrin heavy chain: sequence analysis and restricted localisation in growing pollen tubes.
H. Blackbourn and A. Jackson (1996)
J. Cell Sci. 109, 777-786
   Abstract »    PDF »
The alpha Chain of the AP-2 Adaptor Is a Clathrin Binding Subunit.
O. B. Goodman , Jr. and J. H. Keen (1995)
J. Biol. Chem. 270, 23768-23773
   Abstract »    Full Text »    PDF »
Dynamic or Stable Interactions of Influenza Hemagglutinin Mutants with Coated Pits.
E. Fire, O. Gutman, M. G. Roth, and Y. I. Henis (1995)
J. Biol. Chem. 270, 21075-21081
   Abstract »    Full Text »    PDF »
Roles for a Cytoplasmic Tyrosine and Tyrosine Kinase Activity in the Interactions of Neu Receptors with Coated Pits.
L. Gilboa, R. Ben-Levy, and Y. Yarden (1995)
J. Biol. Chem. 270, 7061-7067
   Abstract »    Full Text »    PDF »
Different Domains of the AP-1 Adaptor Complex Are Required for Golgi Membrane Binding and Clathrin Recruitment.
L. M. Traub, S. Kornfeld, and E. Ungewickell (1995)
J. Biol. Chem. 270, 4933-4942
   Abstract »    Full Text »    PDF »
The Interaction of Calmodulin with Clathrin-coated Vesicles, Triskelions, and Light Chains.
U. M. Pley, B. L. Hill, C. Alibert, F. M. Brodsky, and P. Parham (1995)
J. Biol. Chem. 270, 2395-2402
   Abstract »    Full Text »    PDF »
Saccharomyces cerevisiae Apl2p, a homologue of the mammalian clathrin AP beta subunit, plays a role in clathrin-dependent Golgi functions.
M. Rad, H. Phan, L Kirchrath, P. Tan, T Kirchhausen, C. Hollenberg, and G. Payne (1995)
J. Cell Sci. 108, 1605-1615
   Abstract »    PDF »
Rapid endocytosis of interleukin 2 receptors when clathrin-coated pit endocytosis is inhibited.
A Subtil, A Hemar, and A Dautry-Varsat (1994)
J. Cell Sci. 107, 3461-3468
   Abstract »    PDF »
Brefeldin A causes structural and functional alterations of the trans-Golgi network of MDCK cells.
M Wagner, A. Rajasekaran, D. Hanzel, S Mayor, and E Rodriguez-Boulan (1994)
J. Cell Sci. 107, 933-943
   Abstract »    PDF »
Identification of a beta-type adaptin in plant clathrin-coated vesicles.
S. Holstein, M Drucker, and D. Robinson (1994)
J. Cell Sci. 107, 945-953
   Abstract »    PDF »
unc-101, a gene required for many aspects of Caenorhabditis elegans development and behavior, encodes a clathrin-associated protein..
J Lee, G D Jongeward, and P W Sternberg (1994)
Genes & Dev. 8, 60-73
   Abstract »    PDF »
Interaction of activated EGF receptors with coated pit adaptins.
A Sorkin and G Carpenter (1993)
Science 261, 612-615
   Abstract »    PDF »
Antibodies to clathrin inhibit endocytosis but not recycling to the trans Golgi network in vitro.
R. Draper, Y Goda, F. Brodsky, and S. Pfeffer (1990)
Science 248, 1539-1541
   Abstract »    PDF »
Clathrin: a role in the intracellular retention of a Golgi membrane protein.
G. Payne and R Schekman (1989)
Science 245, 1358-1365
   Abstract »    PDF »
Intracellular Colocalization of Molecules Involved in Antigen Processing and Presentation by B Cells.
F.M. Brodsky, B. Koppelman, J.S. Blum, M.S. Marks, P. Cresswell, and L. Guagliardi (1989)
Cold Spring Harb Symp Quant Biol 54, 319-331
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)