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Science 9 December 1988:
Vol. 242. no. 4884, pp. 1421 - 1423
DOI: 10.1126/science.3144039
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Articles

Science, Vol 242, Issue 4884, 1421-1423
Copyright © 1988 by American Association for the Advancement of Science

articles

Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene

JW Golden and DR Wiest

Department of Biology, Texas A&M University, College Station 77843.

Two genome rearrangements involving 11- and 55-kilobase DNA elements occur during the terminal differentiation of an Anabaena photosynthetic vegetative cell into a nitrogen-fixing heterocyst. The xisA gene, located on the nifD 11-kilobase DNA element, was inactivated by recombination between the chromosome and a copy of the xisA gene that was mutated by inserting an antibiotic gene cassette. Site-directed inactivation of the Anabaena xisA gene blocked rearrangement of the 11-kilobase element and nitrogen fixation, but did not affect rearrangement of the 55-kilobase element, heterocyst differentiation, or heterocyst pattern formation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Anabaena sp. Strain PCC 7120 Gene all2874 Encodes a Diguanylate Cyclase and Is Required for Normal Heterocyst Development under High-Light Growth Conditions.
M. R. Neunuebel and J. W. Golden (2008)
J. Bacteriol. 190, 6829-6836
   Abstract »    Full Text »    PDF »
FraG is necessary for filament integrity and heterocyst maturation in the cyanobacterium Anabaena sp. strain PCC 7120.
A. S. Nayar, H. Yamaura, R. Rajagopalan, D. D. Risser, and S. M. Callahan (2007)
Microbiology 153, 601-607
   Abstract »    Full Text »    PDF »
Regulation by hetC of Genes Required for Heterocyst Differentiation and Cell Division in Anabaena sp. Strain PCC 7120.
Y. Wang and X. Xu (2005)
J. Bacteriol. 187, 8489-8493
   Abstract »    Full Text »    PDF »
Heterocyst-Specific Excision of the Anabaena sp. Strain PCC 7120 hupL Element Requires xisC.
C. D. Carrasco, S. D. Holliday, A. Hansel, P. Lindblad, and J. W. Golden (2005)
J. Bacteriol. 187, 6031-6038
   Abstract »    Full Text »    PDF »
Cytochrome c550 in the Cyanobacterium Thermosynechococcus elongatus: STUDY OF REDOX MUTANTS.
D. Kirilovsky, M. Roncel, A. Boussac, A. Wilson, J. L. Zurita, J.-M. Ducruet, H. Bottin, M. Sugiura, J. M. Ortega, and A. W. Rutherford (2004)
J. Biol. Chem. 279, 52869-52880
   Abstract »    Full Text »    PDF »
Phycobilisome rod mutants in Synechocystis sp. strain PCC6803.
B. Ughy and G. Ajlani (2004)
Microbiology 150, 4147-4156
   Abstract »    Full Text »    PDF »
PsfR, a factor that stimulates psbAI expression in the cyanobacterium Synechococcus elongatus PCC 7942.
C. Thomas, C. R. Andersson, S. R. Canales, and S. S. Golden (2004)
Microbiology 150, 1031-1040
   Abstract »    Full Text »    PDF »
hetL Overexpression Stimulates Heterocyst Formation in Anabaena sp. Strain PCC 7120.
D. Liu and J. W. Golden (2002)
J. Bacteriol. 184, 6873-6881
   Abstract »    Full Text »    PDF »
Identification of the Active Site of HetR Protease and Its Requirement for Heterocyst Differentiation in the Cyanobacterium Anabaena sp. Strain PCC 7120.
Y. Dong, X. Huang, X.-Y. Wu, and J. Zhao (2000)
J. Bacteriol. 182, 1575-1579
   Abstract »    Full Text »
Anabaena xisF gene encodes a developmentally regulated site-specific recombinase..
C D Carrasco, K S Ramaswamy, T S Ramasubramanian, and J W Golden (1994)
Genes & Dev. 8, 74-83
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)