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Science 14 July 1989:
Vol. 245. no. 4914, pp. 180 - 183
DOI: 10.1126/science.2665077
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Articles

Science, Vol 245, Issue 4914, 180-183
Copyright © 1989 by American Association for the Advancement of Science

articles

G1/S transition in normal human T-lymphocytes requires the nuclear protein encoded by c-myb

AM Gewirtz, G Anfossi, D Venturelli, S Valpreda, R Sims, and B Calabretta

Department of Medicine, Temple University School of Medicine, Philadelphia, PA 19140.

Exposure of peripheral blood mononuclear cells (PBMC) to an 18-base c-myb antisense oligomer before mitogen or antigen stimulation resulted in almost complete inhibition of c-myb messenger RNA and protein synthesis and blockade of T lymphocyte proliferation. Expression of early and late activation markers, interleukin-2 receptor and transferrin receptor, respectively, by PBMC was unaffected by antisense oligomer exposure as was the expression of c-myc messenger RNA. In contrast, histone H3 messenger RNA levels and DNA content were selectively decreased. These results suggest that c-myb protein deprivation does not perturb T lymphocyte activation or early molecular events that may prepare the cell for subsequent proliferation. Rather, it appears to specifically block cells in late G1 or early S phase of the cell cycle.


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