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Science 6 October 1989:
Vol. 246. no. 4926, pp. 57 - 63
DOI: 10.1126/science.2675314
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Articles

Science, Vol 246, Issue 4926, 57-63
Copyright © 1989 by American Association for the Advancement of Science

articles

Microcolumn separations and the analysis of single cells

RT Kennedy, MD Oates, BR Cooper, B Nickerson, and JW Jorgenson

Department of Chemistry, University of North Carolina, Chapel Hill 27599.

Capillary zone electrophoresis and open tubular liquid chromatography are two examples of an emerging area of analytical instrumentation known as microcolumn separations. The high resolution and small sample requirements of these methods make them suitable for the quantitative, multicomponent chemical analysis of single cells. Appropriate instrumentation for the analysis of nanoliter and subnanoliter samples is discussed. Data from the analysis of individual neurons are presented, including amino acid and neurotransmitter content.


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