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Science 27 October 1989:
Vol. 246. no. 4929, pp. 488 - 491
DOI: 10.1126/science.2683071
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Articles

Science, Vol 246, Issue 4929, 488-491
Copyright © 1989 by American Association for the Advancement of Science

articles

Human chromosome 12 is required for elevated HIV-1 expression in human-hamster hybrid cells

CE Hart, CY Ou, JC Galphin, J Moore, LT Bacheler, JJ Wasmuth, SR Petteway Jr, and G Schochetman

Centers for Disease Control, Atlanta, GA 30333.

Host cell factors act together with regulatory genes of the human immunodeficiency virus (HIV) to control virus production. Human-Chinese hamster ovary hybrid cell clones were used to probe for human chromosomes involved in regulating HIV gene expression. DNA transfection experiments showed that 4 of 18 clones had high levels of HIV gene expression measured by both extracellular virus production and transactivation of the HIV long terminal repeat in the presence of the trans-activator (tat) gene. Karyotype analyses revealed a 94% concordance (17/18) between human chromosome 12 and HIV gene expression. Other chromosomes had an 11 to 72% concordance with virus production.


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