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Science 27 October 1989:
Vol. 246. no. 4929, pp. 500 - 503
DOI: 10.1126/science.2814477
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Articles

Science, Vol 246, Issue 4929, 500-503
Copyright © 1989 by American Association for the Advancement of Science

articles

Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation

MP King and G Attardi

Division of Biology, California Institute of Technology, Pasadena 91125.

Two human cell lines (termed rho 0), which had been completely depleted of mitochondrial DNA (mtDNA) by long-term exposure to ethidium bromide, were found to be dependent on uridine and pyruvate for growth because of the absence of a functional respiratory chain. Loss of either of these two metabolic requirements was used as a selectable marker for the repopulation of rho 0 cells with exogenous mitochondria by complementation. Transformants obtained with various mitochondrial donors exhibited a respiratory phenotype that was in most cases distinct from that of the rho 0 parent or the donor, indicating that the genotypes of the mitochondrial and nuclear genomes as well as their specific interactions play a role in the respiratory competence of a cell.


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The mtDNA T8993G (NARP) mutation results in an impairment of oxidative phosphorylation that can be improved by antioxidants.
M. Mattiazzi, C. Vijayvergiya, C. D. Gajewski, D. C. DeVivo, G. Lenaz, M. Wiedmann, and G. Manfredi (2004)
Hum. Mol. Genet. 13, 869-879
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Nuclear-Cytoplasmic Interactions Affect In Utero Developmental Capacity, Phenotype, and Cellular Metabolism of Bovine Nuclear Transfer Fetuses.
S. Hiendleder, K. Prelle, K. Bruggerhoff, H.-D. Reichenbach, H. Wenigerkind, D. Bebbere, M. Stojkovic, S. Muller, G. Brem, V. Zakhartchenko, et al. (2004)
Biol Reprod 70, 1196-1205
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Cytochrome c Oxidase Subassemblies in Fibroblast Cultures from Patients Carrying Mutations in COX10, SCO1, or SURF1.
S. L. Williams, I. Valnot, P. Rustin, and J.-W. Taanman (2004)
J. Biol. Chem. 279, 7462-7469
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Resistance of Mitochondrial DNA-depleted Cells against Cell Death: ROLE OF MITOCHONDRIAL SUPEROXIDE DISMUTASE.
S. Y. Park, I. Chang, J.-Y. Kim, S. W. Kang, S.-H. Park, K. Singh, and M.-S. Lee (2004)
J. Biol. Chem. 279, 7512-7520
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