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Science 3 November 1989:
Vol. 246. no. 4930, pp. 661 - 664
DOI: 10.1126/science.2510298
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Articles

Science, Vol 246, Issue 4930, 661-664
Copyright © 1989 by American Association for the Advancement of Science

articles

Initiation by yeast RNA polymerase II at the adenoviral major late promoter in vitro

NF Lue, PM Flanagan, K Sugimoto, and RD Kornberg

Department of Cell Biology, Beckman Laboratories, Fairchild Center, Stanford School of Medicine, CA 94305.

Transcription of the yeast CYC1 promoter fused to a sequence lacking guanosine residues provided a rapid, sensitive assay of initiation by RNA polymerase II in yeast extracts. Initiation was enhanced by yeast and mammalian activator proteins. The adenoviral major late promoter fused to the G-minus sequence was transcribed in yeast extracts with an efficiency comparable to that observed in HeLa extracts, showing that promoters as well as transcription factors are functionally interchangeable across species. Initiation occurred at different sites, approximately 30 and 63 to 69 base pairs downstream of the TATA element of the adenoviral promoter in HeLa and yeast extracts, respectively, distances characteristic of initiation in the two systems in vivo. A component of the transcription system and not the promoter sequence determines the distance to the initiation site.


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DNA melting on yeast RNA polymerase II promoters.
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RNA polymerase II carboxy-terminal domain contributes to the response to multiple acidic activators in vitro..
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A yeast protein that influences the chromatin structure of UASG and functions as a powerful auxiliary gene activator..
D I Chasman, N F Lue, A R Buchman, J W LaPointe, Y Lorch, and R D Kornberg (1990)
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