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Science 10 November 1989:
Vol. 246. no. 4931, pp. 813 - 815
DOI: 10.1126/science.2479099
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Articles

Science, Vol 246, Issue 4931, 813-815
Copyright © 1989 by American Association for the Advancement of Science

articles

Isolation of human transcribed sequences from human-rodent somatic cell hybrids

P Liu, R Legerski, and MJ Siciliano

Department of Molecular Genetics, University of Texas, M.D. Anderson Cancer Center, Houston 77030.

A method was developed for selectively isolating genes from localized regions of the human genome that are contained in interspecific hybrid cells. Complementary human DNA was prepared from a human-rodent somatic cell hybrid that contained less than 1% human DNA, by using consensus 5' intron splice sequences as primers. These primers would select immature, unspliced messenger RNA (still retaining species-specific repeat sequences) as templates. Screening a derived complementary DNA library for human repeat sequences resulted in the isolation of human clones at the anticipated frequency with characteristics expected of exons of transcribed human genes--single copy sequences that hybridized to discrete bands on Northern (RNA) blots.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
An expression-independent catalog of genes from human chromosome 22..
J A Trofatter, K R Long, J R Murrell, C J Stotler, J F Gusella, and A J Buckler (1995)
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Identification of 3'-terminal exons from yeast artificial chromosomes..
D B Krizman, T A Hofmann, U DeSilva, E D Green, P S Meltzer, and J M Trent (1995)
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Direct cloning of human transcripts with HnRNA from hybrid cell lines.
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