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Science 8 December 1989:
Vol. 246. no. 4935, pp. 1300 - 1303
DOI: 10.1126/science.2588006
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Articles

Science, Vol 246, Issue 4935, 1300-1303
Copyright © 1989 by American Association for the Advancement of Science

articles

Cell cycle-dependent regulation of phosphorylation of the human retinoblastoma gene product

K Mihara, XR Cao, A Yen, S Chandler, B Driscoll, AL Murphree, A T'Ang, and YK Fung

Division of Hematology/Oncology and Ophthalmology, Childrens Hospital of Los Angeles, CA.

The human retinoblastoma gene (RB1) encodes a protein (Rb) of 105 kilodaltons that can be phosphorylated. Analysis of Rb metabolism has shown that the protein has a half-life of more than 10 hours and is synthesized at all phases of the cell cycle. Newly synthesized Rb is not extensively phosphorylated (it is "underphosphorylated") in cells in the G0 and G1 phases but is phosphorylated at multiple sites at the G1/S boundary and in S phase. HL-60 cells that were induced to terminally differentiate by various chemicals lost their ability to phosphorylate newly synthesized Rb at multiple sites when cell growth was arrested. These findings suggest that underphosphorylated Rb may restrict cell proliferation.


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Failure to phosphorylate the retinoblastoma gene product in senescent human fibroblasts.
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Involvement of pRB-related p107 Protein in the Inhibition of S Phase Progression in Response to Genotoxic Stress.
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