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Science 8 December 1989:
Vol. 246. no. 4935, pp. 1306 - 1309
DOI: 10.1126/science.2479986

Articles

Science, Vol 246, Issue 4935, 1306-1309
Copyright © 1989 by American Association for the Advancement of Science


articles

Vascular endothelial growth factor is a secreted angiogenic mitogen

DW Leung, G Cachianes, WJ Kuang, DV Goeddel, and N Ferrara

Department of Molecular Biology, Genetech, South San Francisco, CA 94080.

Vascular endothelial growth factor (VEGF) was purified from media conditioned by bovine pituitary folliculostellate cells (FC). VEGF is a heparin-binding growth factor specific for vascular endothelial cells that is able to induce angiogenesis in vivo. Complementary DNA clones for bovine and human VEGF were isolated from cDNA libraries prepared from FC and HL60 leukemia cells, respectively. These cDNAs encode hydrophilic proteins with sequences related to those of the A and B chains of platelet-derived growth factor. DNA sequencing suggests the existence of several molecular species of VEGF. VEGFs are secreted proteins, in contrast to other endothelial cell mitogens such as acidic or basic fibroblast growth factors and platelet-derived endothelial cell growth factor. Human 293 cells transfected with an expression vector containing a bovine or human VEGF cDNA insert secrete an endothelial cell mitogen that behaves like native VEGF.


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Model of Vasculogenesis from Embryonic Stem Cells for Vascular Research and Regenerative Medicine.
H. Nakagami, N. Nakagawa, Y. Takeya, K. Kashiwagi, C. Ishida, S.-i. Hayashi, M. Aoki, K. Matsumoto, T. Nakamura, T. Ogihara, et al. (2006)
Hypertension 48, 112-119
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Vascular endothelial growth factor is an important determinant of sepsis morbidity and mortality.
K. Yano, P. C. Liaw, J. M. Mullington, S.-C. Shih, H. Okada, N. Bodyak, P. M. Kang, L. Toltl, B. Belikoff, J. Buras, et al. (2006)
J. Exp. Med. 203, 1447-1458
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Hepatocyte Growth Factor Induces Retinal Vascular Permeability via MAP-Kinase and PI-3 Kinase without Altering Retinal Hemodynamics..
A. C. Clermont, M. Cahill, H. Salti, S. L. Rook, C. Rask-Madsen, L. Goddard, J. S. Wong, D. Bursell, S. E. Bursell, and L. P. Aiello (2006)
Invest. Ophthalmol. Vis. Sci. 47, 2701-2708
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Differential effects of selective endothelin type a receptor antagonist on the gene expression of vascular endothelial growth factor and its receptors in streptozotocin-induced diabetic heart..
S. Jesmin, S. Zaedi, N. Yamaguchi, S. Maeda, N. Shimojo, K. Masuzawa, I. Yamaguchi, K. Goto, and T. Miyauchi (2006)
Experimental Biology and Medicine 231, 902-906
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A Novel Noninvasive Model of Endometriosis for Monitoring the Efficacy of Antiangiogenic Therapy.
C. M. Becker, R. D. Wright, R. Satchi-Fainaro, T. Funakoshi, J. Folkman, A. L. Kung, and R. J. D'Amato (2006)
Am. J. Pathol. 168, 2074-2084
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Neovascularization in Human Atherosclerosis.
P. R. Moreno, K-R. Purushothaman, M. Sirol, A. P. Levy, and V. Fuster (2006)
Circulation 113, 2245-2252
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The Role of PDGF Receptor Inhibitors and PI3-Kinase Signaling in the Pathogenesis of Corneal Neovascularization.
S. Dell, S. Peters, P. Muther, N. Kociok, and A. M. Joussen (2006)
Invest. Ophthalmol. Vis. Sci. 47, 1928-1937
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