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Science 22 December 1989:
Vol. 246. no. 4937, pp. 1585 - 1587
DOI: 10.1126/science.2595370
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Articles

Science, Vol 246, Issue 4937, 1585-1587
Copyright © 1989 by American Association for the Advancement of Science

articles

Volatilization of high molecular weight DNA by pulsed laser ablation of frozen aqueous solutions

RW Nelson, MJ Rainbow, DE Lohr, and P Williams

Department of Chemistry, Arizona State University, Tempe 85287.

DNA has been volatilized by pulsed laser ablation of a thin film of a frozen aqueous DNA solution. The target film was irradiated in vacuum by a pulsed laser at power densities sufficient to ablate the ice matrix. The expanding ablated water vapor entrained embedded DNA molecules and expelled them into the gas phase. Ejection of DNA molecules as large as 410 kilodaltons was verified by collection of the ablation products and subsequent mass analysis by polyacrylamide gel electrophoresis with autoradiographic detection.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)