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Science 29 June 1990:
Vol. 248. no. 4963, pp. 1625 - 1630
DOI: 10.1126/science.2363050
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Articles

Science, Vol 248, Issue 4963, 1625-1630
Copyright © 1990 by American Association for the Advancement of Science

articles

Functional domains and upstream activation properties of cloned human TATA binding protein

MG Peterson, N Tanese, BF Pugh, and R Tjian

Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.

The TATA binding protein, TFIID, plays a central role in the initiation of eukaryotic mRNA synthesis. Here, we present a human cDNA clone for this factor. Comparison of its predicted protein sequence with those from Drosophila and yeast reveals a highly conserved carboxyl-terminal 180 amino acids. By contrast, the amino-terminal region of TFIID has diverged in both sequence and length. A striking feature of the human protein is a stretch of 38 glutamine residues in the NH2-terminal region. Expression of human TFIID in both Escherichia coli and HeLa cells produces a protein that binds specifically to a TATA box and promotes basal transcription; the conserved COOH-terminal portion of the protein is sufficient for both of these activities. Recombinant TFIID forms a stable complex on a TATA box either alone or in combination with either of the general transcription factors, TFIIA or TFIIB. Full-length recombinant TFIID is able to support Sp1 activated transcription in a TFIID-depleted nuclear extract, while a deletion of the NH2-terminal half of the protein is not. These results indicate the importance of the NH2-terminal region for upstream activation functions and suggest that additional factors (co-activators) are required for mediating interactions with specific regulators.


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M E Maxon, J A Goodrich, and R Tjian (1994)
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   Abstract »    PDF »
Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex..
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   Abstract »    PDF »
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   Abstract »    PDF »
Cooperative interactions between the Caenorhabditis elegans homeoproteins UNC-86 and MEC-3.
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Science 261, 1324-1328
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Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE..
C L Sadowski, R W Henry, S M Lobo, and N Hernandez (1993)
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Identification of a minimal set of proteins that is sufficient for accurate initiation of transcription by RNA polymerase II..
C M Tyree, C P George, L M Lira-DeVito, S L Wampler, M E Dahmus, L Zawel, and J T Kadonaga (1993)
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TBP, a universal eukaryotic transcription factor?.
N Hernandez (1993)
Genes & Dev. 7, 1291-1308
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Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID..
T Kokubo, D W Gong, S Yamashita, M Horikoshi, R G Roeder, and Y Nakatani (1993)
Genes & Dev. 7, 1033-1046
   Abstract »    PDF »
Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain..
Q Zhou, T G Boyer, and A J Berk (1993)
Genes & Dev. 7, 180-187
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Coactivators and TAFs: A New Class of Eukaryotic Transcription Factors That Connect Activators to the Basal Machinery.
N. Tanese and R. Tjian (1993)
Cold Spring Harb Symp Quant Biol 58, 179-185
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The Cycling of RNA Polymerase II during Transcription.
L. Zawel, H. Lu, L.J. Cisek, J.L. Corden, and D. Reinberg (1993)
Cold Spring Harb Symp Quant Biol 58, 187-198
   Abstract »    PDF »
Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter..
Q Zhou, P M Lieberman, T G Boyer, and A J Berk (1992)
Genes & Dev. 6, 1964-1974
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Cloning of the 62-kilodalton component of basic transcription factor BTF2.
L Fischer, M Gerard, C Chalut, Y Lutz, S Humbert, M Kanno, P Chambon, and J. Egly (1992)
Science 257, 1392-1395
   Abstract »    PDF »
Isolation of two genes that encode subunits of the yeast transcription factor IIA.
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Science 255, 1127-1129
   Abstract »    PDF »
Transcription factor IID mutants defective for interaction with transcription factor IIA.
S Buratowski and H Zhou (1992)
Science 255, 1130-1132
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Yeast and human TFIIDs are interchangeable for the response to acidic transcriptional activators in vitro..
R J Kelleher, P M Flanagan, D I Chasman, A S Ponticelli, K Struhl, and R D Kornberg (1992)
Genes & Dev. 6, 296-303
   Abstract »    PDF »
TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters..
J Colgan and J L Manley (1992)
Genes & Dev. 6, 304-315
   Abstract »    PDF »
Coactivators for a proline-rich activator purified from the multisubunit human TFIID complex..
N Tanese, B F Pugh, and R Tjian (1991)
Genes & Dev. 5, 2212-2224
   Abstract »    PDF »
The Zta trans-activator protein stabilizes TFIID association with promoter DNA by direct protein-protein interaction..
P M Lieberman and A J Berk (1991)
Genes & Dev. 5, 2441-2454
   Abstract »    PDF »
Transcription from a TATA-less promoter requires a multisubunit TFIID complex..
B F Pugh and R Tjian (1991)
Genes & Dev. 5, 1935-1945
   Abstract »    PDF »
The mammalian TFIID protein is present in two functionally distinct complexes..
H T Timmers and P A Sharp (1991)
Genes & Dev. 5, 1946-1956
   Abstract »    PDF »
The functional BPV-1 E2 trans-activating protein can act as a repressor by preventing formation of the initiation complex..
N Dostatni, P F Lambert, R Sousa, J Ham, P M Howley, and M Yaniv (1991)
Genes & Dev. 5, 1657-1671
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The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro..
S M Lobo, J Lister, M L Sullivan, and N Hernandez (1991)
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   Abstract »    PDF »
Assembly of Partial TFIID Complexes in Mammalian Cells Reveals Distinct Activities Associated with Individual TATA Box-binding Protein-associated Factors.
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   Abstract »    Full Text »    PDF »


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