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Science 13 July 1990:
Vol. 249. no. 4965, pp. 157 - 160
DOI: 10.1126/science.2115209
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Articles

Science, Vol 249, Issue 4965, 157-160
Copyright © 1990 by American Association for the Advancement of Science

articles

Solution structure of the glucocorticoid receptor DNA-binding domain

T Hard, E Kellenbach, R Boelens, BA Maler, K Dahlman, LP Freedman, J Carlstedt-Duke, KR Yamamoto, JA Gustafsson, and R Kaptein

Department of Chemistry, University of Utrecht, The Netherlands.

The three-dimensional structure of the DNA-binding domain (DBD) of the glucocorticoid receptor has been determined by nuclear magnetic resonance spectroscopy and distance geometry. The structure of a 71-residue protein fragment containing two "zinc finger" domains is based on a large set of proton-proton distances derived from nuclear Overhauser enhancement spectra, hydrogen bonds in previously identified secondary structure elements, and coordination of two zinc atoms by conserved cysteine residues. The DBD is found to consist of a globular body from which the finger regions extend. A model of the dimeric complex between the DBD and the glucocorticoid response element is proposed. The model is consistent with previous results indicating that specific amino acid residues of the DBD are involved in protein-DNA and protein-protein interactions.


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