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Science 27 July 1990:
Vol. 249. no. 4967, pp. 404 - 406
DOI: 10.1126/science.2143033
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Articles

Science, Vol 249, Issue 4967, 404-406
Copyright © 1990 by American Association for the Advancement of Science

articles

Random peptide libraries: a source of specific protein binding molecules

JJ Devlin, LC Panganiban, and PE Devlin

Department of Molecular Biology, Cetus Corporation, Emeryville, CA 94608.

Libraries of random peptide sequences were constructed and screened to identify peptides that specifically bind to proteins. In one of these about 2 X 10(7) different 15-residue peptide sequences were expressed on the surface of the coliphage M13. Each phage encoded a single random sequence and expressed it as a fusion complex with pIII, a minor coat protein present at five molecules per phage. Phage encoding nine different streptavidin-binding peptide sequences were isolated from this library. The core consensus sequence was His-Pro-Gln and binding of these phage to streptavidin was inhibited by biotin. This type of library makes it possible to identify peptides that bind to proteins (or other macromolecules) that have no previously known affinity for peptides.


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J. Biol. Chem. 270, 16974-16980
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Science 269, 66-69
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A Combinatorial Approach to Materials Discovery.
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Science 268, 1738-1740
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Characterization of S-100b Binding Epitopes.
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J. Biol. Chem. 270, 14651-14658
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Rapid Identification of Highly Active and Selective Substrates for Stromelysin and Matrilysin Using Bacteriophage Peptide Display Libraries.
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Expanding the scope of RNA catalysis.
Prudent JR, T Uno, and P. Schultz (1994)
Science 264, 1924-1927
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Randomization of genes by PCR mutagenesis..
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Genome Res. 2, 28-33
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A combinatorial approach toward DNA recognition.
D. Pei, H. Ulrich, and P. Schultz (1991)
Science 253, 1408-1411
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Light-directed, spatially addressable parallel chemical synthesis.
S. Fodor, J. Read, M. Pirrung, L Stryer, A. Lu, and D Solas (1991)
Science 251, 767-773
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Searching for peptide ligands with an epitope library.
J. Scott and G. Smith (1990)
Science 249, 386-390
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Reaction Kinetics of Protease with Substrate Phage. KINETIC MODEL DEVELOPED USING STROMELYSIN.
N. A. Sharkov, R. M. Davis, J. F. Reidhaar-Olson, M. Navre, and D. Cai (2001)
J. Biol. Chem. 276, 10788-10793
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T. Huberman, Y. Eisenberg-Domovich, G. Gitlin, T. Kulik, E. A. Bayer, M. Wilchek, and O. Livnah (2001)
J. Biol. Chem. 276, 32031-32039
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