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Science 3 August 1990:
Vol. 249. no. 4968, pp. 518 - 521
DOI: 10.1126/science.2382132
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Articles

Science, Vol 249, Issue 4968, 518-521
Copyright © 1990 by American Association for the Advancement of Science

articles

Structural transitions upon ligand binding in a cooperative dimeric hemoglobin

WE Royer Jr, WA Hendrickson, and E Chiancone

Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.

Comparison of the 2.4 angstrom resolution crystal structures of dimeric clam hemoglobin in the deoxygenated and carbon-monoxide liganded states shows how radically different the structural basis for cooperative oxygen binding is from that operative in mammalian hemoglobins. Heme groups are in direct communication across a novel subunit interface formed by the E and F helices. The conformational changes at this interface that accompany ligand binding are more dramatic at a tertiary level but more subtle at a quaternary level than those in mammalian hemoglobins. These findings suggest a cooperative mechanism that links ligation at one subunit with potentiation of affinity at the second subunit.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Functional and Biochemical Properties of the Hemoglobins of the Burrowing Brittle Star Hemipholis elongata Say (Echinodermata, Ophiuroidea).
A. B. Christensen, J. M. Colacino, and C. Bonaventura (2003)
Biol. Bull. 205, 54-65
   Abstract »    Full Text »    PDF »
The Mutation K30D Disrupts the Only Salt Bridge at the Subunit Interface of the Homodimeric Hemoglobin from Scapharca inaequivalvis and Changes the Mechanism of Cooperativity.
P. Ceci, L. Giangiacomo, A. Boffi, and E. Chiancone (2002)
J. Biol. Chem. 277, 6929-6933
   Abstract »    Full Text »    PDF »
Nonvertebrate Hemoglobins: Functions and Molecular Adaptations.
R. E. Weber and S. N. Vinogradov (2001)
Physiol Rev 81, 569-628
   Abstract »    Full Text »    PDF »
Hydroxide Rather Than Histidine Is Coordinated to the Heme in Five-coordinate Ferric Scapharca inaequivalvis Hemoglobin.
T. K. Das, A. Boffi, E. Chiancone, and D. L. Rousseau (1999)
J. Biol. Chem. 274, 2916-2919
   Abstract »    Full Text »    PDF »
Ligand Linked Assembly of Scapharca Dimeric Hemoglobin.
W. E. Royer Jr., R. A. Fox, F. R. Smith, D. Zhu, and E. H. Braswell (1997)
J. Biol. Chem. 272, 5689-5694
   Abstract »    Full Text »    PDF »
Cooperative Oxygen Binding to Scapharca inaequivalvis Hemoglobin in the Crystal.
A. Mozzarelli, S. Bettati, C. Rivetti, G. L. Rossi, G. Colotti, and E. Chiancone (1996)
J. Biol. Chem. 271, 3627-3632
   Abstract »    Full Text »    PDF »


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