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Science 3 August 1990:
Vol. 249. no. 4968, pp. 533 - 537
DOI: 10.1126/science.2166335

Articles

Science, Vol 249, Issue 4968, 533-537
Copyright © 1990 by American Association for the Advancement of Science


articles

Identification of a sequence in the PEPCK gene that mediates a negative effect of insulin on transcription

RM O'Brien, PC Lucas, CD Forest, MA Magnuson, and DK Granner

Department of Molecular Physiology and Biophysics, Vanderbilt University Medical School, Nashville, TN 37232-0615.

Phosphoenolpyruvate carboxykinase (PEPCK) governs the rate-limiting step in gluconeogenesis. Glucocorticoids and adenosine 3',5'-monophosphate (cAMP) increase PEPCK gene transcription and gluconeogenesis, whereas insulin has the opposite effect. Insulin is dominant, since it prevents cAMP and glucocorticoid-stimulated transcription. Glucocorticoid and cAMP response elements have been located in the PEPCK gene and now a 15-base pair insulin-responsive sequence (IRS) is described. Evidence for a binding activity that recognizes this sequence is presented.


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Identification of an Insulin-responsive Element in the Rat Insulin-like Growth Factor-binding Protein-3 Gene.
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J. Biol. Chem. 271, 17296-17303
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Cloning and Characterization of a Functional Promoter of the Rat pp120 Gene, Encoding a Substrate of the Insulin Receptor Tyrosine Kinase.
S. M. Najjar, Y. R. Boisclair, Z. T. Nabih, N. Philippe, Y. Imai, Y. Suzuki, D.-S. Suh, and G. T. Ooi (1996)
J. Biol. Chem. 271, 8809-8817
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Adenovirus E1A Proteins Regulate Phosphoenolpyruvate Carboxykinase Gene Transcription through Multiple Mechanisms.
D. J. Klemm, L. A. Colton, S. Ryan, and J. M. Routes (1996)
J. Biol. Chem. 271, 8082-8088
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Identification and Characterization of a Second Retinoic Acid Response Element in the Phosphoenolpyruvate Carboxykinase Gene Promoter.
D. K. Scott, J. A. Mitchell, and D. K. Granner (1996)
J. Biol. Chem. 271, 6260-6264
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Receptors Coupled to Pertussis Toxin-sensitive G-proteins Traffic to Opposite Surfaces in Madin-Darby Canine Kidney Cells.
C. Saunders, J. R. Keefer, A. P. Kennedy, J. N. Wells, and L. E. Limbird (1996)
J. Biol. Chem. 271, 995-1002
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Transcriptional Modulators Affect in Vivo Protein Binding to the Low Density Lipoprotein Receptor and 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Promoters.
D. B. Lloyd and J. F. Thompson (1995)
J. Biol. Chem. 270, 25812-25818
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Phosphatidylinositol 3-Kinase, but Not p70/p85 Ribosomal S6 Protein Kinase, Is Required for the Regulation of Phosphoenolpyruvate Carboxykinase (PEPCK) Gene Expression by Insulin.
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J. Biol. Chem. 270, 15501-15506
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cAMP-associated Inhibition of Phenobarbital-inducible Cytochrome P450 Gene Expression in Primary Rat Hepatocyte Cultures.
J. S. Sidhu and C. J. Omiecinski (1995)
J. Biol. Chem. 270, 12762-12773
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Endogenous retroviral sequences are required for tissue-specific expression of a human salivary amylase gene..
C N Ting, M P Rosenberg, C M Snow, L C Samuelson, and M H Meisler (1992)
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Insulin Responsiveness of the Glucagon Gene Conferred by Interactions between Proximal Promoter and More Distal Enhancer-like Elements Involving the Paired-domain Transcription Factor Pax6.
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NF-kappa B Inhibits Glucocorticoid and cAMP-mediated Expression of the Phosphoenolpyruvate Carboxykinase Gene.
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   Abstract »    Full Text »    PDF »
The Use of Transgenic Mice to Analyze the Role of Accessory Factor Two in the Regulation of Phosphoenolpyruvate Carboxykinase (GTP) Gene Transcription during Diabetes.
P. S. Lechner, C. M. Croniger, P. Hakimi, C. Millward, C. Fekter, J. S. Yun, and R. W. Hanson (2001)
J. Biol. Chem. 276, 22675-22679
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Sterol Regulatory Element-binding Protein-1c Mimics the Negative Effect of Insulin on Phosphoenolpyruvate Carboxykinase (GTP) Gene Transcription.
K. Chakravarty, P. Leahy, D. Becard, P. Hakimi, M. Foretz, P. Ferre, F. Foufelle, and R. W. Hanson (2001)
J. Biol. Chem. 276, 34816-34823
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Transducin-like Enhancer of Split Proteins, the Human Homologs of Drosophila Groucho, Interact with Hepatic Nuclear Factor 3beta.
J.-C. Wang, M. Waltner-Law, K. Yamada, H. Osawa, S. Stifani, and D. K. Granner (2000)
J. Biol. Chem. 275, 18418-18423
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