Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 10 August 1990:
Vol. 249. no. 4969, pp. 652 - 655
DOI: 10.1126/science.2382140
Prev | Table of Contents | Next

Articles

Science, Vol 249, Issue 4969, 652-655
Copyright © 1990 by American Association for the Advancement of Science

articles

Direct cloning of human transcripts with HnRNA from hybrid cell lines

L Corbo, JA Maley, DL Nelson, and CT Caskey

Institute for Molecular Genetics, Baylor College of Medicine, Houston, TX 77030.

A library of human-derived complementary DNA from a human-hamster hybrid cell line containing the Xq24-qter region has been constructed. Complementary DNA synthesis was primed from heterogeneous nuclear (hn) RNA by oligonucleotides derived from conserved regions of human Alu repeats. At least 80% of these cloned sequences were of human origin, providing an enrichment of at least two orders of magnitude. Two clones, one containing a fragment of the primary transcript of the human hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene at Xq26 and another recognizing a family of human genes mapping to two regions of Xq24-qter, were characterized. Additional hncDNA clones mapped to a variety of sites in the Xq24-qter region, demonstrating the isolation of many transcriptionally active loci. These clones provide probes for identification of genetic loci on the terminal region of the X chromosome long arm, which is the location of a number of inherited disorders.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
An expression-independent catalog of genes from human chromosome 22..
J A Trofatter, K R Long, J R Murrell, C J Stotler, J F Gusella, and A J Buckler (1995)
Genome Res. 5, 214-224
   Abstract »    PDF »
Optimization of long-distance PCR using a transposon-based model system..
L D Ohler and E A Rose (1992)
Genome Res. 2, 51-59
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)