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Science 17 August 1990:
Vol. 249. no. 4970, pp. 802 - 804
DOI: 10.1126/science.1975122
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Articles

Science, Vol 249, Issue 4970, 802-804
Copyright © 1990 by American Association for the Advancement of Science

articles

Autoradiographic imaging of phosphoinositide turnover in the brain

PM Hwang, DS Bredt, and SH Snyder

Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

With [3H]cytidine as a precursor, phosphoinositide turnover can be localized in brain slices by selective autoradiography of the product [3H]cytidine diphosphate diacylglycerol, which is membrane-bound. In the cerebellum, glutamatergic stimulation elicits an increase of phosphoinositide turnover only in Purkinje cells and the molecular layer. In the hippocampus, both glutamatergic and muscarinic cholinergic stimulation increase phosphoinositide turnover, but with distinct localizations. Cholinergic stimulation affects CA1, CA3, CA4, and subiculum, whereas glutamatergic effects are restricted to the subiculum and CA3. Imaging phosphoinositide turnover in brain slices, which are amenable to electrophysiologic studies, will permit a dynamic localized analysis of regulation of this second messenger in response to synaptic stimulation of specific neuronal pathways.


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