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Science 31 August 1990:
Vol. 249. no. 4972, pp. 1033 - 1037
DOI: 10.1126/science.2168579
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Articles

Science, Vol 249, Issue 4972, 1033-1037
Copyright © 1990 by American Association for the Advancement of Science

articles

Molecular cloning and functional expression of glutamate receptor subunit genes

J Boulter, M Hollmann, A O'Shea-Greenfield, M Hartley, E Deneris, C Maron, and S Heinemann

Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, San Diego, CA 92138.

Three closely related genes, GluR1, GluR2, and GluR3, encode receptor subunits for the excitatory neurotransmitter glutamate. The proteins encoded by the individual genes form homomeric ion channels in Xenopus oocytes that are sensitive to glutamatergic agonists such as kainate and quisqualate but not to N-methyl-D-aspartate, indicating that binding sites for kainate and quisqualate exist on single receptor polypeptides. In addition, kainate-evoked conductances are potentiated in oocytes expressing two or more of the cloned receptor subunits. Electrophysiological responses obtained with certain subunit combinations show agonist profiles and current-voltage relations that are similar to those obtained in vivo. Finally, in situ hybridization histochemistry reveals that these genes are transcribed in shared neuroanatomical loci. Thus, as with gamma-aminobutyric acid, glycine, and nicotinic acetylcholine receptors, native kainate-quisqualate-sensitive glutamate receptors form a family of heteromeric proteins.


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{blacksquare} REVIEW : Glutamate Receptors: Emerging Links Between Subunit Proteins and Specific Excitatory Circuits in Primate Hippocampus and Neocortex.
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Organization of AMPA Receptor Subunits at a Glutamate Synapse: A Quantitative Immunogold Analysis of Hair Cell Synapses in the Rat Organ of Corti.
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Glutamate Receptor Autoimmunity in Rasmussen's Encephalitis.
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The GluR2 Hypothesis: Ca++-permeable AMPA Receptors in Delayed Neurodegeneration.
M.V.L. Bennett, D.E. Pellegrini-Giampietro, J.A. Gorter, E. Aronica, J.A. Connor, and R.S. Zukin (1996)
Cold Spring Harb Symp Quant Biol 61, 373-384
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Autoantibodies to glutamate receptor GluR3 in Rasmussen's encephalitis.
S. Rogers, P. Andrews, L. Gahring, T Whisenand, K Cauley, B Crain, T. Hughes, S. Heinemann, and J. McNamara (1994)
Science 265, 648-651
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Differential changes in glutamate receptor subunit messenger RNAs in rat brain after haloperidol treatment.
S. L. Eastwood, P. Story, P. W. J. Burnet, P. Heath, and P. J. Harrison (1994)
J Psychopharmacol 8, 196-203
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Molecular diversity of glutamate receptors and implications for brain function.
S Nakanishi (1992)
Science 258, 597-603
   Abstract »    PDF »
Control by asparagine residues of calcium permeability and magnesium blockade in the NMDA receptor.
N Burnashev, R Schoepfer, H Monyer, J. Ruppersberg, W Gunther, P. Seeburg, and B Sakmann (1992)
Science 257, 1415-1419
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Calcium-permeable AMPA-kainate receptors in fusiform cerebellar glial cells.
N Burnashev, A Khodorova, P Jonas, P. Helm, W Wisden, H Monyer, P. Seeburg, and B Sakmann (1992)
Science 256, 1566-1570
   Abstract »    PDF »
Heteromeric NMDA Receptors: Molecular and Functional Distinction of Subtypes.
H. Monyer, R. Sprengel, R. Schoepfer, A. Herb, M. Higuchi, H. Lomeli, N. Burnashev, B. Sakmann, and P. H. Seeburg (1992)
Science 256, 1217-1221
   Abstract »    PDF »
Molecular cloning of an invertebrate glutamate receptor subunit expressed in Drosophila muscle.
C. Schuster, A Ultsch, P Schloss, J. Cox, B Schmitt, and H Betz (1991)
Science 254, 112-114
   Abstract »    PDF »
Identification of a site in glutamate receptor subunits that controls calcium permeability.
R. Hume, R Dingledine, and S. Heinemann (1991)
Science 253, 1028-1031
   Abstract »    PDF »
Structural determinants of ion flow through recombinant glutamate receptor channels.
T. Verdoorn, N Burnashev, H Monyer, P. Seeburg, and B Sakmann (1991)
Science 252, 1715-1718
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Cloning, expression, and gene structure of a G protein-coupled glutamate receptor from rat brain.
K. Houamed, J. Kuijper, T. Gilbert, B. Haldeman, P. O'Hara, E. Mulvihill, W Almers, and F. Hagen (1991)
Science 252, 1318-1321
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Ca2+ permeability of KA-AMPA--gated glutamate receptor channels depends on subunit composition.
M Hollmann, M Hartley, and S Heinemann (1991)
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Permeation of calcium ions through non-NMDA glutamate channels in retinal bipolar cells.
T. Gilbertson, R Scobey, and M Wilson (1991)
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The Na+/Ca2+ Exchanger NCX1 Has Oppositely Oriented Reentrant Loop Domains That Contain Conserved Aspartic Acids Whose Mutation Alters Its Apparent Ca2+ Affinity.
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