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Science 14 September 1990:
Vol. 249. no. 4974, pp. 1281 - 1285
DOI: 10.1126/science.2205002
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Articles

Science, Vol 249, Issue 4974, 1281-1285
Copyright © 1990 by American Association for the Advancement of Science

articles

Fragments of the HIV-1 Tat protein specifically bind TAR RNA

KM Weeks, C Ampe, SC Schultz, TA Steitz, and DM Crothers

Department of Chemistry, Yale University, New Haven, CT.

Proteolytically produced carboxyl-terminal fragments of the human immunodeficiency virus type-1 (HIV-1) Tat protein that include a conserved region rich in arginine and lysine bind specifically to transactivation response RNA sequences (TAR). A chemically synthesized 14-residue peptide spanning the basic subdomain also recognizes TAR, identifying this subdomain as central for RNA interaction. TAR RNA forms a stable hairpin that includes a six-residue loop, a trinucleotide pyrimidine bulge, and extensive duplex structure. Competition and interference experiments show that the Tat-derived fragments bind to double-stranded RNA and interact specifically at the pyrimidine bulge and adjacent duplex of TAR.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Protein localization to the nucleolus: a search for targeting domains in nucleolin.
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The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence..
L S Tiley, S J Madore, M H Malim, and B R Cullen (1992)
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Pseudo--half-knot formation with RNA.
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Conformation of the TAR RNA-arginine complex by NMR spectroscopy.
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H Kato, H Sumimoto, P Pognonec, C H Chen, C A Rosen, and R G Roeder (1992)
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C D Southgate and M R Green (1991)
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Two distinct nuclear transcription factors recognize loop and bulge residues of the HIV-1 TAR RNA hairpin..
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F Wu, J Garcia, D Sigman, and R Gaynor (1991)
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Analysis of arginine-rich peptides from the HIV Tat protein reveals unusual features of RNA-protein recognition..
B J Calnan, S Biancalana, D Hudson, and A D Frankel (1991)
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Synergy between HIV-1 Tat and adenovirus E1A is principally due to stabilization of transcriptional elongation..
M F Laspia, A P Rice, and M B Mathews (1990)
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