Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 14 September 1990:
Vol. 249. no. 4974, pp. 1291 - 1293
DOI: 10.1126/science.2205003
Prev | Table of Contents | Next

Articles

Science, Vol 249, Issue 4974, 1291-1293
Copyright © 1990 by American Association for the Advancement of Science

articles

Differentiation of mouse erythroleukemia cells enhanced by alternatively spliced c-myb mRNA

BL Weber, EH Westin, and MF Clarke

Department of Internal Medicine, University of Michigan School of Medicine, Ann Arbor 48109.

C-myb, the normal cellular homolog of the retroviral transforming gene v-myb, encodes a nuclear, transcriptional regulatory protein (p75c-myb). C-myb is involved in regulating normal human hematopoiesis, and inhibits dimethyl sulfoxide-induced differentiation of Friend murine erythroleukemia (F-MEL) cells. An alternately spliced c-myb mRNA encodes a truncated version of p75c-myb (mbm2) that includes the DNA binding region and nuclear localization signal present in the c-myb protein, but does not contain the transcriptional regulatory regions. Constitutive expression of mbm2, in contrast to c-myb, here resulted in enhanced differentiation of F-MEL cells. These data suggest that the c-myb protooncogene encodes alternately spliced mRNA species with opposing effects on differentiation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
CREB-binding protein and p300: molecular integrators of hematopoietic transcription.
G. A. Blobel (2000)
Blood 95, 745-755
   Full Text »    PDF »
Fli-1, an Ets-Related Transcription Factor, Regulates Erythropoietin-Induced Erythroid Proliferation and Differentiation: Evidence for Direct Transcriptional Repression of the Rb Gene during Differentiation.
A. Tamir, J. Howard, R. R. Higgins, Y.-J. Li, L. Berger, E. Zacksenhaus, M. Reis, and Y. Ben-David (1999)
Mol. Cell. Biol. 19, 4452-4464
   Abstract »    Full Text »    PDF »
Expression of the alpha(1,3)Fucosyltransferase Fuc-TVII in Lymphoid Aggregate High Endothelial Venules Correlates with Expression of L-Selectin Ligands.
P. L. Smith, K. M. Gersten, B. Petryniak, R. J. Kelly, C. Rogers, Y. Natsuka, J. A. Alford III, E. P. Scheidegger, S. Natsuka, and J. B. Lowe (1996)
J. Biol. Chem. 271, 8250-8259
   Abstract »    Full Text »    PDF »
Nuclear proteins of the bovine esophageal epithelium. II. The NuMA gene gives rise to multiple mRNAs and gene products reactive with monoclonal antibody W1.
T. Tang, C. Tang, Y. Chen, and C. Wu (1993)
J. Cell Sci. 104, 249-260
   Abstract »    PDF »
A dominant negative form of transcription activator mTFE3 created by differential splicing.
C Roman, L Cohn, and K Calame (1991)
Science 254, 94-97
   Abstract »    PDF »
Specific DNA binding by c-Myb: evidence for a double helix-turn-helix-related motif.
O. Gabrielsen, A Sentenac, and P Fromageot (1991)
Science 253, 1140-1143
   Abstract »    PDF »
Alternative splicing of fosB transcripts results in differentially expressed mRNAs encoding functionally antagonistic proteins..
D Mumberg, F C Lucibello, M Schuermann, and R Muller (1991)
Genes & Dev. 5, 1212-1223
   Abstract »    PDF »
New light on Myc and Myb. Part II. Myb..
B Luscher and R N Eisenman (1990)
Genes & Dev. 4, 2235-2241
   PDF »
The Product of the Rice myb7 Unspliced mRNA Dimerizes with the Maize Leucine Zipper Opaque2 and Stimulates Its Activity in a Transient Expression Assay.
F. Locatelli, M. Bracale, F. Magaraggia, F. Faoro, L. A. Manzocchi, and I. Coraggio (2000)
J. Biol. Chem. 275, 17619-17625
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)