Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 5 October 1990:
Vol. 250. no. 4977, pp. 94 - 98
DOI: 10.1126/science.2218515
Prev | Table of Contents | Next

Articles

Science, Vol 250, Issue 4977, 94-98
Copyright © 1990 by American Association for the Advancement of Science

articles

Chromosomal region of the cystic fibrosis gene in yeast artificial chromosomes: a model for human genome mapping

ED Green and MV Olson

Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110.

A general strategy for cloning and mapping large regions of human DNA with yeast artificial chromosomes (YAC's) is described. It relies on the use of the polymerase chain reaction to detect DNA landmarks called sequence-tagged sites (STS's) within YAC clones. The method was applied to the region of human chromosome 7 containing the cystic fibrosis (CF) gene. Thirty YAC clones from this region were analyzed, and a contig map that spans more than 1,500,000 base pairs was assembled. Individual YAC's as large as 790 kilobase pairs and containing the entire CF gene were constructed in vivo by meiotic recombination in yeast between pairs of overlapping YAC's.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Ancient haplotypes of the HLA Class II region.
C. K. Raymond, A. Kas, M. Paddock, R. Qiu, Y. Zhou, S. Subramanian, J. Chang, A. Palmieri, E. Haugen, R. Kaul, et al. (2005)
Genome Res. 15, 1250-1257
   Abstract »    Full Text »    PDF »
Human chromosome 7 circa 2004: a model for structural and functional studies of the human genome.
S. W. Scherer and E. D. Green (2004)
Hum. Mol. Genet. 13, R303-R313
   Abstract »    Full Text »    PDF »
A High-Resolution Radiation Hybrid Map of the Human Genome Draft Sequence.
M. Olivier, A. Aggarwal, J. Allen, A. A. Almendras, E. S. Bajorek, E. M. Beasley, S. D. Brady, J. M. Bushard, V. I. Bustos, A. Chu, et al. (2001)
Science 291, 1298-1302
   Abstract »    Full Text »
Comparative Genome Mapping in the Sequence-based Era: Early Experience with Human Chromosome 7.
J. W. Thomas, T. J. Summers, S.-Q. Lee-Lin, V. V. B. Maduro, J. R. Idol, S. D. Mastrian, J. F. Ryan, D. C. Jamison, and E. D. Green (2000)
Genome Res. 10, 624-633
   Abstract »    Full Text »
A Minimal Critical Region of the 8p22-23 Amplicon in Esophageal Adenocarcinomas Defined Using Sequence Tagged Site-Amplification Mapping and Quantitative Polymerase Chain Reaction Includes the GATA-4 Gene.
L. Lin, S. Aggarwal, T. W. Glover, M. B. Orringer, S. Hanash, and D. G. Beer (2000)
Cancer Res. 60, 1341-1347
   Abstract »    Full Text »
Comparative genomic sequence analysis of the human and mouse cystic fibrosis transmembrane conductance regulator genes.
R. E. Ellsworth, D. C. Jamison, J. W. Touchman, S. L. Chissoe, V. V. Braden Maduro, G. G. Bouffard, N. L. Dietrich, S. M. Beckstrom-Sternberg, L. M. Iyer, L. A. Weintraub, et al. (2000)
PNAS 97, 1172-1177
   Abstract »    Full Text »    PDF »
Sequence-tagged connectors: A sequence approach to mapping and scanning the human genome.
G. G. Mahairas, J. C. Wallace, K. Smith, S. Swartzell, T. Holzman, A. Keller, R. Shaker, J. Furlong, J. Young, S. Zhao, et al. (1999)
PNAS 96, 9739-9744
   Abstract »    Full Text »    PDF »
Error Checking and Graphical Representation of Multiple-Complete-Digest (MCD) Restriction-Fragment Maps.
E. C. Thayer, M. V. Olson, and R. M. Karp (1999)
Genome Res. 9, 79-90
   Abstract »    Full Text »
Toward a Complete Human Genome Sequence.
T. S. Centre and T. W. U. G. S. Center (1998)
Genome Res. 8, 1097-1108
   Abstract »    Full Text »
Construction and validation of yeast artificial chromosome contig maps by RecA-assisted restriction endonuclease cleavage.
P. Lauer, S. S. Schneider, and A. Gnirke (1998)
PNAS 95, 11318-11323
   Abstract »    Full Text »    PDF »
Masquerading Repeats: Paralogous Pitfalls of the Human Genome.
E. E. Eichler (1998)
Genome Res. 8, 758-762
   Full Text »
Integrated YAC Contig Map of the Prader-Willi/Angelman Region on Chromosome 15q11-q13 with Average STS Spacing of 35 kb.
S. L. Christian, N. K. Bhatt, S. A. Martin, J. S. Sutcliffe, T. Kubota, B. Huang, A. Mutirangura, A. C. Chinault, A. L. Beaudet, and D. H. Ledbetter (1998)
Genome Res. 8, 146-157
   Abstract »    Full Text »
Sequence Ready---or Not?.
J. D. McPherson (1997)
Genome Res. 7, 1111-1113
   Full Text »    PDF »
A Physical Map of Human Chromosome 7: An Integrated YAC Contig Map with Average STS Spacing of 79 kb.
G. G. Bouffard, J. R. Idol, V. V. Braden, L. M. Iyer, A. F. Cunningham, L. A. Weintraub, J. W. Touchman, R. M. Mohr-Tidwell, D. C. Peluso, R. S. Fulton, et al. (1997)
Genome Res. 7, 673-692
   Abstract »    Full Text »    PDF »
Molecular linguistics: Extracting information from gene and protein sequences.
D. Botstein and J. M. Cherry (1997)
PNAS 94, 5506-5507
   Full Text »    PDF »
Multiple-complete-digest restriction fragment mapping: Generating sequence-ready maps for large-scale DNA sequencing.
G. K.-S. Wong, J. Yu, E. C. Thayer, and M. V. Olson (1997)
PNAS 94, 5225-5230
   Abstract »    Full Text »    PDF »
An STS-Based Radiation Hybrid Map of the Human Genome.
E. A. Stewart, K. B. McKusick, A. Aggarwal, E. Bajorek, S. Brady, A. Chu, N. Fang, D. Hadley, M. Harris, S. Hussain, et al. (1997)
Genome Res. 7, 422-433
   Abstract »    Full Text »    PDF »
X chromosome map at 75-kb STS resolution, revealing extremes of recombination and GC content..
R Nagaraja, S MacMillan, J Kere, C Jones, S Griffin, M Schmatz, J Terrell, M Shomaker, C Jermak, C Hott, et al. (1997)
Genome Res. 7, 210-222
   Abstract »    PDF »
A collection of 1814 human chromosome 7-specific STSs..
G G Bouffard, L M Iyer, J R Idol, V V Braden, A F Cunningham, L A Weintraub, R M Mohr-Tidwell, D C Peluso, R S Fulton, M P Leckie, et al. (1997)
Genome Res. 7, 59-64
   Abstract »    PDF »
An STS-Based Map of the Human Genome.
T. J. Hudson, L. D. Stein, S. S. Gerety, J. Ma, A. B. Castle, J. Silva, D. K. Slonim, R. Baptista, L. Kruglyak, S.-H. Xu, et al. (1995)
Science 270, 1945-1954
   Abstract »
A Time to Sequence.
M. V. Olson (1995)
Science 270, 394
   Abstract »    PDF »
Assessing mapping progress in the Human Genome Project.
D. Cox, E. Green, E. Lander, D Cohen, and R. Myers (1994)
Science 265, 2031-2032
   PDF »
Forward and reverse genetic approaches to behavior in the mouse.
J. Takahashi, L. Pinto, and M. Vitaterna (1994)
Science 264, 1724-1733
   Abstract »    PDF »
Isolating vector-insert junctions from yeast artificial chromosomes..
G A Silverman (1993)
Genome Res. 3, 141-150
   PDF »
Criterion for the Completeness of Large-scale Physical Maps of DNA.
M.V. Olson and P. Green (1993)
Cold Spring Harb Symp Quant Biol 58, 349-355
   Abstract »    PDF »
The human Y chromosome: overlapping DNA clones spanning the euchromatic region.
S Foote, D Vollrath, A Hilton, and D. Page (1992)
Science 258, 60-66
   Abstract »    PDF »
Sequence-tagged site (STS) content mapping of human chromosomes: theoretical considerations and early experiences..
E D Green and P Green (1991)
Genome Res. 1, 77-90
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)