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Science 29 November 1991:
Vol. 254. no. 5036, pp. 1388 - 1390
DOI: 10.1126/science.1962198

Articles

Science, Vol 254, Issue 5036, 1388-1390
Copyright © 1991 by American Association for the Advancement of Science


articles

Prevention of apoptosis by a baculovirus gene during infection of insect cells

RJ Clem, M Fechheimer, and LK Miller

Department of Genetics, University of Georgia, Athens 30602.

Programmed cell death is an active process of self destruction that is important in both the development and maintenance of multicellular animals. The molecular mechanisms controlling activation or suppression of programmed cell death are largely unknown. Apoptosis, a morphologically and biochemically defined type of programmed cell death commonly seen in vertebrates, was found to be initiated during baculovirus replication in insect cells. A specific viral gene product, p35, was identified as being responsible for blocking the apoptotic response. Identification of the function of this gene will allow further definition of the molecular pathways involved in the regulation of programmed cell death and may identify the role of apoptosis in invertebrate viral defense systems.


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Adenovirus-Mediated Gene Expression In Vivo Is Enhanced by the Antiapoptotic Bcl-2 Gene.
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The Apoptosis Inhibitor Gene API2 and a Novel 18q Gene, MLT, Are Recurrently Rearranged in the t(11;18)(q21;q21) Associated With Mucosa-Associated Lymphoid Tissue Lymphomas.
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Role for Caspase-Mediated Cleavage of Rad51 in Induction of Apoptosis by DNA Damage.
Y. Huang, S. Nakada, T. Ishiko, T. Utsugisawa, R. Datta, S. Kharbanda, K. Yoshida, R. V. Talanian, R. Weichselbaum, D. Kufe, et al. (1999)
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C. J. Hawkins, S. L. Wang, and B. A. Hay (1999)
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A Mutational Analysis of the Baculovirus Inhibitor of Apoptosis Op-IAP.
D. Vucic, W. J. Kaiser, and L. K. Miller (1998)
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Nineteen Baculovirus Open Reading Frames, Including LEF-12, Support Late Gene Expression.
J. C. Rapp, J. A. Wilson, and L. K. Miller (1998)
J. Virol. 72, 10197-10206
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Persistent Baculovirus Infection Results from Deletion of the Apoptotic Suppressor Gene p35.
J.-C. Lee, H.-H. Chen, and Y.-C. Chao (1998)
J. Virol. 72, 9157-9165
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Role of the Fas/Fas Ligand Apoptotic Pathway in Human Immunodeficiency Virus Type 1 Disease.
D. Kaplan and S. Sieg (1998)
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A Giant Ubiquitin-conjugating Enzyme Related to IAP Apoptosis Inhibitors.
H.-P. Hauser, M. Bardroff, G. Pyrowolakis, and S. Jentsch (1998)
J. Cell Biol. 141, 1415-1422
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J.-F. Eleouet, S. Chilmonczyk, L. Besnardeau, and H. Laude (1998)
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D. Vucic, W. J. Kaiser, and L. K. Miller (1998)
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M. Resnicoff, B. Valentinis, D. Herbert, D. Abraham, P. D. Friesen, E. S. Alnemri, and R. Baserga (1998)
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M. Itoh, H. Hotta, and M. Homma (1998)
J. Virol. 72, 2927-2934
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Evidence of a Role for Phosphatidylinositol 3-Kinase Activation in the Blocking of Apoptosis by Polyomavirus Middle T Antigen.
J. Dahl, A. Jurczak, L. A. Cheng, D. C. Baker, and T. L. Benjamin (1998)
J. Virol. 72, 3221-3226
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Essential contribution of caspase 3/CPP32 to apoptosis and its associated nuclear changes.
M. Woo, R. Hakem, M. S. Soengas, G. S. Duncan, A. Shahinian, D. Kägi, A. Hakem, M. McCurrach, W. Khoo, S. A. Kaufman, et al. (1998)
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Membrane Oligomerization and Cleavage Activates the Caspase-8 (FLICE/MACHalpha 1) Death Signal.
D. A. Martin, R. M. Siegel, L. Zheng, and M. J. Lenardo (1998)
J. Biol. Chem. 273, 4345-4349
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Toxoplasma gondii-Infected Cells Are Resistant to Multiple Inducers of Apoptosis.
P. B. Nash, M. B. Purner, R. P. Leon, P. Clarke, R. C. Duke, and T. J. Curiel (1998)
J. Immunol. 160, 1824-1830
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Human IAP-Like Protein Regulates Programmed Cell Death Downstream of Bcl-xL and Cytochrome c.
C. S. Duckett, F. Li, Y. Wang, K. J. Tomaselli, C. B. Thompson, and R. C. Armstrong (1998)
Mol. Cell. Biol. 18, 608-615
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Baculovirus inhibitors of apoptosis (IAPs) block activation of Sf-caspase-1.
S. Seshagiri and L. K. Miller (1997)
PNAS 94, 13606-13611
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CD30-dependent degradation of TRAF2: implications for negative regulation of TRAF signaling and the control of cell survival.
C. S. Duckett and C. B. Thompson (1997)
Genes & Dev. 11, 2810-2821
   Abstract »    Full Text »    PDF »
Activation of distinct caspase-like proteases by Fas and reaper in Drosophila cells.
T. Kondo, T. Yokokura, and S. Nagata (1997)
PNAS 94, 11951-11956
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Activation of CPP32-Like Caspases Contributes to Neuronal Apoptosis and Neurological Dysfunction after Traumatic Brain Injury.
A. G. Yakovlev, S. M. Knoblach, L. Fan, G. B. Fox, R. Goodnight, and A. I. Faden (1997)
J. Neurosci. 17, 7415-7424
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Caspase Cleavage of Keratin 18 and Reorganization of Intermediate Filaments during Epithelial Cell Apoptosis.
C. Caulin, G. S. Salvesen, and R. G. Oshima (1997)
J. Cell Biol. 138, 1379-1394
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Inhibition of Reaper-induced apoptosis by interaction with inhibitor of apoptosis proteins (IAPs).
D. Vucic, W. J. Kaiser, A. J. Harvey, and L. K. Miller (1997)
PNAS 94, 10183-10188
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A Study of the Interferon Antiviral Mechanism: Apoptosis Activation by the 2-5A System.
J. C. Castelli, B. A. Hassel, K. A. Wood, X.-L. Li, K. Amemiya, M. C. Dalakas, P. F. Torrence, and R. J. Youle (1997)
J. Exp. Med. 186, 967-972
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CASH, a Novel Caspase Homologue with Death Effector Domains.
Y. V. Goltsev, A. V. Kovalenko, E. Arnold, E. E. Varfolomeev, V. M. Brodianskii, and D. Wallach (1997)
J. Biol. Chem. 272, 19641-19644
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A Bcl-2 homolog encoded by Kaposi sarcoma-associated virus, human herpesvirus 8, inhibits apoptosis but does not heterodimerize with Bax or Bak.
E. H.-Y. Cheng, J. Nicholas, D. S. Bellows, G. S. Hayward, H.-G. Guo, M. S. Reitz, and J. M. Hardwick (1997)
PNAS 94, 690-694
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Spodoptera frugiperda Caspase-1, a Novel Insect Death Protease That Cleaves the Nuclear Immunophilin FKBP46, Is the Target of the Baculovirus Antiapoptotic Protein p35.
M. Ahmad, S. M. Srinivasula, L. Wang, G. Litwack, T. Fernandes-Alnemri, and E. S. Alnemri (1997)
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R. Datta, H. Kojima, D. Banach, N. J. Bump, R. V. Talanian, E. S. Alnemri, R. R. Weichselbaum, W. W. Wong, and D. W. Kufe (1997)
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Inhibition of interleukin 1beta -converting enzyme-mediated apoptosis of mammalian cells by baculovirus IAP.
C. J. Hawkins, A. G. Uren, G. Hacker, R. L. Medcalf, and D. L. Vaux (1996)
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grim, a novel cell death gene in Drosophila..
P Chen, W Nordstrom, B Gish, and J M Abrams (1996)
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   Abstract »    PDF »
Fas-induced Activation of the Cell Death-related Protease CPP32 Is Inhibited by Bcl-2 and by ICE Family Protease Inhibitors.
R. C. Armstrong, T. Aja, J. Xiang, S. Gaur, J. F. Krebs, K. Hoang, X. Bai, S. J. Korsmeyer, D. S. Karanewsky, L. C. Fritz, et al. (1996)
J. Biol. Chem. 271, 16850-16855
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REVIEW {blacksquare} : Keeping Neurons Alive: The Molecular Control of Apoptosis (Part II.
D. E. Bredesen (1996)
Neuroscientist 2, 211-216
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REVIEW {blacksquare} : Keeping Neurons Alive: The Molecular Control of Apoptosis (Part I.
D. E. Bredesen (1996)
Neuroscientist 2, 181-190
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The Caenorhabditis elegans cell-death protein CED-3 is a cysteine protease with substrate specificities similar to those of the human CPP32 protease..
D Xue, S Shaham, and H R Horvitz (1996)
Genes & Dev. 10, 1073-1083
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