Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Science Signaling

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 6 December 1991:
Vol. 254. no. 5037, pp. 1512 - 1515
DOI: 10.1126/science.1660188
Prev | Table of Contents | Next

Articles

Science, Vol 254, Issue 5037, 1512-1515
Copyright © 1991 by American Association for the Advancement of Science

articles

Regulation of phagocyte oxygen radical production by the GTP-binding protein Rac 2

UG Knaus, PG Heyworth, T Evans, JT Curnutte, and GM Bokoch

Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.

A major action of the microbicidal system of human neutrophils is the formation of superoxide anion (O2-) by a multicomponent oxidase that transfers electrons from the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) to molecular oxygen. The mechanism of assembly and activation of the oxidase from its cytosolic and membrane-bound components is unknown, but may require the activity of a guanosine 5'-triphosphate (GTP)-binding component. A cytosolic GTP-binding protein (Gox) that regulates the NADPH oxidase of neutrophils was identified. Gox was purified and shown to augment the rate of O2- production in a cell-free oxidase activation system. Sequence analysis of peptide fragments from Gox identified it as Rac 2, a member of the Ras superfamily of GTP-binding proteins. Antibody to a peptide derived from the COOH-terminus of Rac 2 inhibited O2- generation in a concentration-dependent manner. These results suggest that Rac 2 is a regulatory component of the human neutrophil NADPH oxidase, and provide new insights into the mechanism by which this oxygen radical-generating system is regulated.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification of Proteins Binding to E-Box/Ku86 Sites and Function of the Tumor Suppressor SAFB1 in Transcriptional Regulation of the Human Xanthine Oxidoreductase Gene.
J. Lin, P. Xu, P. LaVallee, and J. R. Hoidal (2008)
J. Biol. Chem. 283, 29681-29689
   Abstract »    Full Text »    PDF »
Identification of a Conserved Rac-binding Site on NADPH Oxidases Supports a Direct GTPase Regulatory Mechanism.
Y.-Y. Kao, D. Gianni, B. Bohl, R. M. Taylor, and G. M. Bokoch (2008)
J. Biol. Chem. 283, 12736-12746
   Abstract »    Full Text »    PDF »
Rap1a Null Mice Have Altered Myeloid Cell Functions Suggesting Distinct Roles for the Closely Related Rap1a and 1b Proteins.
Y. Li, J. Yan, P. De, H.-C. Chang, A. Yamauchi, K. W. Christopherson II, N. C. Paranavitana, X. Peng, C. Kim, V. Munugulavadla, et al. (2007)
J. Immunol. 179, 8322-8331
   Abstract »    Full Text »    PDF »
Rac2 GTPase activation by angiotensin II is modulated by Ca2+/calcineurin and mitogen-activated protein kinases in human neutrophils.
R. El Bekay, G. Alba, M E. Reyes, P. Chacon, A. Vega, J. Martin-Nieto, J. Jimenez, E. Ramos, J. Olivan, E. Pintado, et al. (2007)
J. Mol. Endocrinol. 39, 351-363
   Abstract »    Full Text »    PDF »
Vav proteins control MyD88-dependent oxidative burst.
A. V. Miletic, D. B. Graham, V. Montgrain, K. Fujikawa, T. Kloeppel, K. Brim, B. Weaver, R. Schreiber, R. Xavier, and W. Swat (2007)
Blood 109, 3360-3368
   Abstract »    Full Text »    PDF »
A Regulated Adaptor Function of p40phox: Distinct p67phox Membrane Targeting by p40phox and by p47phox.
T. Ueyama, T. Tatsuno, T. Kawasaki, S. Tsujibe, Y. Shirai, H. Sumimoto, T. L. Leto, and N. Saito (2007)
Mol. Biol. Cell 18, 441-454
   Abstract »    Full Text »    PDF »
The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology.
K. Bedard and K.-H. Krause (2007)
Physiol Rev 87, 245-313
   Abstract »    Full Text »    PDF »
Rac1 mediates intestinal epithelial cell apoptosis via JNK.
S. Jin, R. M. Ray, and L. R. Johnson (2006)
Am J Physiol Gastrointest Liver Physiol 291, G1137-G1147
   Abstract »    Full Text »    PDF »
Retinal microglial activation and chemotaxis by docosahexaenoic Acid hydroperoxide..
S. Saraswathy, G. Wu, and N. A. Rao (2006)
Invest. Ophthalmol. Vis. Sci. 47, 3656-3663
   Abstract »    Full Text »    PDF »
Reactive oxygen species in vascular endothelial cell motility. Roles of NAD(P)H oxidase and Rac1.
L. Moldovan, K. Mythreye, P. J. Goldschmidt-Clermont, and L. L. Satterwhite (2006)
Cardiovasc Res 71, 236-246
   Abstract »    Full Text »    PDF »
Liposomes Comprising Anionic but Not Neutral Phospholipids Cause Dissociation of Rac(1 or 2){middle dot}RhoGDI Complexes and Support Amphiphile-independent NADPH Oxidase Activation by Such Complexes.
Y. Ugolev, S. Molshanski-Mor, C. Weinbaum, and E. Pick (2006)
J. Biol. Chem. 281, 19204-19219
   Abstract »    Full Text »    PDF »
Activation State-Dependent Interaction between G{alpha}i and p67phox..
C. Marty, T. Kozasa, M. T. Quinn, and R. D. Ye (2006)
Mol. Cell. Biol. 26, 5190-5200
   Abstract »    Full Text »    PDF »
Nox1-dependent Reactive Oxygen Generation Is Regulated by Rac1.
G. Cheng, B. A. Diebold, Y. Hughes, and J. D. Lambeth (2006)
J. Biol. Chem. 281, 17718-17726
   Abstract »    Full Text »    PDF »
Involvement of rac1 in activation of multicomponent nox1- and nox3-based NADPH oxidases..
T. Ueyama, M. Geiszt, and T. L. Leto (2006)
Mol. Cell. Biol. 26, 2160-2174
   Abstract »    Full Text »    PDF »
Signaling Mechanisms Regulating Endothelial Permeability.
D. Mehta and A. B. Malik (2006)
Physiol Rev 86, 279-367
   Abstract »    Full Text »    PDF »
p21-activated kinase (Pak) regulates NADPH oxidase activation in human neutrophils.
K. D. Martyn, M.-J. Kim, M. T. Quinn, M. C. Dinauer, and U. G. Knaus (2005)
Blood 106, 3962-3969
   Abstract »    Full Text »    PDF »
Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation.
F. R. Sheppard, M. R. Kelher, E. E. Moore, N. J. D. McLaughlin, A. Banerjee, and C. C. Silliman (2005)
J. Leukoc. Biol. 78, 1025-1042
   Abstract »    Full Text »    PDF »
Isoform-Specific Membrane Targeting Mechanism of Rac during Fc{gamma}R-Mediated Phagocytosis: Positive Charge-Dependent and Independent Targeting Mechanism of Rac to the Phagosome.
T. Ueyama, M. Eto, K. Kami, T. Tatsuno, T. Kobayashi, Y. Shirai, M. R. Lennartz, R. Takeya, H. Sumimoto, and N. Saito (2005)
J. Immunol. 175, 2381-2390
   Abstract »    Full Text »    PDF »
Calcium-sensing soluble adenylyl cyclase mediates TNF signal transduction in human neutrophils.
H. Han, A. Stessin, J. Roberts, K. Hess, N. Gautam, M. Kamenetsky, O. Lou, E. Hyde, N. Nathan, W. A. Muller, et al. (2005)
J. Exp. Med. 202, 353-361
   Abstract »    Full Text »    PDF »
Superoxide Production in Galleria mellonella Hemocytes: Identification of Proteins Homologous to the NADPH Oxidase Complex of Human Neutrophils.
D. Bergin, E. P. Reeves, J. Renwick, F. B. Wientjes, and K. Kavanagh (2005)
Infect. Immun. 73, 4161-4170
   Abstract »    Full Text »    PDF »
Generation and Characterization of Rac3 Knockout Mice.
S. Corbetta, S. Gualdoni, C. Albertinazzi, S. Paris, L. Croci, G. G. Consalez, and I. de Curtis (2005)
Mol. Cell. Biol. 25, 5763-5776
   Abstract »    Full Text »    PDF »
Myeloperoxidase: friend and foe.
S. J. Klebanoff (2005)
J. Leukoc. Biol. 77, 598-625
   Abstract »    Full Text »    PDF »
Activation of the Phagocyte NADPH Oxidase by Rac Guanine Nucleotide Exchange Factors in Conjunction with ATP and Nucleoside Diphosphate Kinase.
A. Mizrahi, S. Molshanski-Mor, C. Weinbaum, Y. Zheng, M. Hirshberg, and E. Pick (2005)
J. Biol. Chem. 280, 3802-3811
   Abstract »    Full Text »    PDF »
Helicobacter pylori lipopolysaccharide activates Rac1 and transcription of NADPH oxidase Nox1 and its organizer NOXO1 in guinea pig gastric mucosal cells.
T. Kawahara, M. Kohjima, Y. Kuwano, H. Mino, S. Teshima-Kondo, R. Takeya, S. Tsunawaki, A. Wada, H. Sumimoto, and K. Rokutan (2005)
Am J Physiol Cell Physiol 288, C450-C457
   Abstract »    Full Text »    PDF »
Reconstitution of Chemotactic Peptide-Induced Nicotinamide Adenine Dinucleotide Phosphate (Reduced) Oxidase Activation in Transgenic COS-phox Cells.
R. He, M. Nanamori, H. Sang, H. Yin, M. C. Dinauer, and R. D. Ye (2004)
J. Immunol. 173, 7462-7470
   Abstract »    Full Text »    PDF »
Important role for Rac1 in regulating reactive oxygen species generation and pulmonary arterial smooth muscle cell growth.
S. Patil, M. Bunderson, J. Wilham, and S. M. Black (2004)
Am J Physiol Lung Cell Mol Physiol 287, L1314-L1322
   Abstract »    Full Text »    PDF »
Rac2-Deficient Murine Macrophages Have Selective Defects in Superoxide Production and Phagocytosis of Opsonized Particles.
A. Yamauchi, C. Kim, S. Li, C. C. Marchal, J. Towe, S. J. Atkinson, and M. C. Dinauer (2004)
J. Immunol. 173, 5971-5979
   Abstract »    Full Text »    PDF »
Rac2 is critical for neutrophil primary granule exocytosis.
D. Abdel-Latif, M. Steward, D. L. Macdonald, G. A. Francis, M. C. Dinauer, and P. Lacy (2004)
Blood 104, 832-839
   Abstract »    Full Text »    PDF »
Antagonistic Cross-talk between Rac and Cdc42 GTPases Regulates Generation of Reactive Oxygen Species.
B. A. Diebold, B. Fowler, J. Lu, M. C. Dinauer, and G. M. Bokoch (2004)
J. Biol. Chem. 279, 28136-28142
   Abstract »    Full Text »    PDF »
Rac1 and Toll-IL-1 Receptor Domain-Containing Adapter Protein Mediate Toll-Like Receptor 4 Induction of HIV-Long Terminal Repeat.
O. Equils, Z. Madak, C. Liu, K. S. Michelsen, Y. Bulut, and D. Lu (2004)
J. Immunol. 172, 7642-7646
   Abstract »    Full Text »    PDF »
The Arabidopsis Rab GTPase RabA4b Localizes to the Tips of Growing Root Hair Cells.
M. L. Preuss, J. Serna, T. G. Falbel, S. Y. Bednarek, and E. Nielsen (2004)
PLANT CELL 16, 1589-1603
   Abstract »    Full Text »    PDF »
Characterization of Proteins Binding to E-box/Ku86 Sites and Function of Ku86 in Transcriptional Regulation of the Human Xanthine Oxidoreductase Gene.
P. Xu, P. A. LaVallee, J. J. Lin, and J. R. Hoidal (2004)
J. Biol. Chem. 279, 16057-16063
   Abstract »    Full Text »    PDF »
G Protein {beta}{gamma} Subunits Stimulate p114RhoGEF, a Guanine Nucleotide Exchange Factor for RhoA and Rac1: Regulation of Cell Shape and Reactive Oxygen Species Production.
J. Niu, J. Profirovic, H. Pan, R. Vaiskunaite, and T. Voyno-Yasenetskaya (2003)
Circ. Res. 93, 848-856
   Abstract »    Full Text »    PDF »
Hematopoietic Cell Regulation by Rac1 and Rac2 Guanosine Triphosphatases.
Y. Gu, M.-D. Filippi, J. A. Cancelas, J. E. Siefring, E. P. Williams, A. C. Jasti, C. E. Harris, A. W. Lee, R. Prabhakar, S. J. Atkinson, et al. (2003)
Science 302, 445-449
   Abstract »    Full Text »    PDF »
Human Monocytes Use Rac1, Not Rac2, in the NADPH Oxidase Complex.
X. Zhao, K. A. Carnevale, and M. K. Cathcart (2003)
J. Biol. Chem. 278, 40788-40792
   Abstract »    Full Text »    PDF »
Activation of Rac2 and Cdc42 on Fc and complement receptor ligation in human neutrophils.
M. Forsberg, P. Druid, L. Zheng, O. Stendahl, and E. Sarndahl (2003)
J. Leukoc. Biol. 74, 611-619
   Abstract »    Full Text »    PDF »
Rac regulates cardiovascular superoxide through diverse molecular interactions: more than a binary GTP switch.
D. Gregg, F. M. Rauscher, and P. J. Goldschmidt-Clermont (2003)
Am J Physiol Cell Physiol 285, C723-C734
   Abstract »    Full Text »    PDF »
Reactive oxygen species as essential mediators of cell adhesion: the oxidative inhibition of a FAK tyrosine phosphatase is required for cell adhesion.
P. Chiarugi, G. Pani, E. Giannoni, L. Taddei, R. Colavitti, G. Raugei, M. Symons, S. Borrello, T. Galeotti, and G. Ramponi (2003)
J. Cell Biol. 161, 933-944
   Abstract »    Full Text »    PDF »
Rac1 Deletion in Mouse Neutrophils Has Selective Effects on Neutrophil Functions.
M. Glogauer, C. C. Marchal, F. Zhu, A. Worku, B. E. Clausen, I. Foerster, P. Marks, G. P. Downey, M. Dinauer, and D. J. Kwiatkowski (2003)
J. Immunol. 170, 5652-5657
   Abstract »    Full Text »    PDF »
The Small GTP-binding Protein Rac1 Induces Cardiac Myocyte Hypertrophy through the Activation of Apoptosis Signal-regulating Kinase 1 and Nuclear Factor-{kappa}B.
Y. Higuchi, K. Otsu, K. Nishida, S. Hirotani, H. Nakayama, O. Yamaguchi, S. Hikoso, K. Kashiwase, T. Takeda, T. Watanabe, et al. (2003)
J. Biol. Chem. 278, 20770-20777
   Abstract »    Full Text »    PDF »
Divergence of Mechanisms Regulating Respiratory Burst in Blood and Sputum Eosinophils and Neutrophils from Atopic Subjects.
P. Lacy, D. A. Latif, M. Steward, S. Musat-Marcu, S. F. P. Man, and R. Moqbel (2003)
J. Immunol. 170, 2670-2679
   Abstract »    Full Text »    PDF »
Critical Role of the Carboxyl Terminus of Proline-rich Tyrosine Kinase (Pyk2) in the Activation of Human Neutrophils by Tumor Necrosis Factor: Separation of Signals for the Respiratory Burst and Degranulation.
H. Han, M. Fuortes, and C. Nathan (2003)
J. Exp. Med. 197, 63-75
   Abstract »    Full Text »    PDF »
Repression of rac2 mRNA Expression by Anaplasma phagocytophila Is Essential to the Inhibition of Superoxide Production and Bacterial Proliferation.
J. A. Carlyon, W.-T. Chan, J. Galan, D. Roos, and E. Fikrig (2002)
J. Immunol. 169, 7009-7018
   Abstract »    Full Text »    PDF »
Preferential and Asymmetrical Accumulation of a Rac Small GTPase mRNA in Differentiating Xylem Cells of Zinnia elegans.
I. Nakanomyo, B. Kost, N.-H. Chua, and H. Fukuda (2002)
Plant Cell Physiol. 43, 1484-1492
   Abstract »    Full Text »    PDF »
Chemoattractant-Stimulated Rac Activation in Wild-Type and Rac2-Deficient Murine Neutrophils: Preferential Activation of Rac2 and Rac2 Gene Dosage Effect on Neutrophil Functions.
S. Li, A. Yamauchi, C. C. Marchal, J. K. Molitoris, L. A. Quilliam, and M. C. Dinauer (2002)
J. Immunol. 169, 5043-5051
   Abstract »    Full Text »    PDF »
The HIV-1 Nef Protein and Phagocyte NADPH Oxidase Activation.
F. Vilhardt, O. Plastre, M. Sawada, K. Suzuki, M. Wiznerowicz, E. Kiyokawa, D. Trono, and K.-H. Krause (2002)
J. Biol. Chem. 277, 42136-42143
   Abstract »    Full Text »    PDF »
A p21-Activated Kinase-controlled Metabolic Switch Up-regulates Phagocyte NADPH Oxidase.
T. Shalom-Barak and U. G. Knaus (2002)
J. Biol. Chem. 277, 40659-40665
   Abstract »    Full Text »    PDF »
Activation of Rac-1, Rac-2, and Cdc42 by hemopoietic growth factors or cross-linking of the B-lymphocyte receptor for antigen.
B. Grill and J. W. Schrader (2002)
Blood 100, 3183-3192
   Abstract »    Full Text »    PDF »
Current molecular models for NADPH oxidase regulation by Rac GTPase.
G. M. Bokoch and B. A. Diebold (2002)
Blood 100, 2692-2695
   Abstract »    Full Text »    PDF »
The TRQQKRP motif located near the C-terminus of Rac2 is essential for Rac2 biologic functions and intracellular localization.
W. Tao, M.-D. Filippi, J. R. Bailey, S. J. Atkinson, B. Connors, A. Evan, and D. A. Williams (2002)
Blood 100, 1679-1688
   Abstract »    Full Text »    PDF »
Rac Activation Induces NADPH Oxidase Activity in Transgenic COSphox Cells, and the Level of Superoxide Production Is Exchange Factor-dependent.
M. O. Price, S. J. Atkinson, U. G. Knaus, and M. C. Dinauer (2002)
J. Biol. Chem. 277, 19220-19228
   Abstract »    Full Text »    PDF »
Creation of a genetic system for analysis of the phagocyte respiratory burst: high-level reconstitution of the NADPH oxidase in a nonhematopoietic system.
M. O. Price, L. C. McPhail, J. D. Lambeth, C.-H. Han, U. G. Knaus, and M. C. Dinauer (2002)
Blood 99, 2653-2661
   Abstract »    Full Text »    PDF »
Merlin Phosphorylation by p21-activated Kinase 2 and Effects of Phosphorylation on Merlin Localization.
J. L. Kissil, K. C. Johnson, M. S. Eckman, and T. Jacks (2002)
J. Biol. Chem. 277, 10394-10399
   Abstract »    Full Text »    PDF »
Deficiency of Small GTPase Rac2 Affects T Cell Activation.
H. Yu, D. Leitenberg, B. Li, and R. A. Flavell (2001)
J. Exp. Med. 194, 915-926
   Abstract »    Full Text »    PDF »
IL-8 activates endothelial cell CXCR1 and CXCR2 through Rho and Rac signaling pathways.
I. U. Schraufstatter, J. Chung, and M. Burger (2001)
Am J Physiol Lung Cell Mol Physiol 280, L1094-L1103
   Abstract »    Full Text »    PDF »
Antagonists of Calcium Fluxes and Calmodulin Block Activation of the p21-Activated Protein Kinases in Neutrophils.
J. P. Lian, L. Crossley, Q. Zhan, R. Huang, P. Coffer, A. Toker, D. Robinson, and J. A. Badwey (2001)
J. Immunol. 166, 2643-2650
   Abstract »    Full Text »    PDF »
Rac2 Is an Essential Regulator of Neutrophil Nicotinamide Adenine Dinucleotide Phosphate Oxidase Activation in Response to Specific Signaling Pathways.
C. Kim and M. C. Dinauer (2001)
J. Immunol. 166, 1223-1232
   Abstract »    Full Text »    PDF »
Differential Localization of Rho GTPases in Live Cells: Regulation by Hypervariable Regions and RhoGDI Binding.
D. Michaelson, J. Silletti, G. Murphy, P. D'Eustachio, M. Rush, and M. R. Philips (2001)
J. Cell Biol. 152, 111-126
   Abstract »    Full Text »    PDF »
Small GTP-Binding Proteins.
Y. Takai, T. Sasaki, and T. Matozaki (2001)
Physiol Rev 81, 153-208
   Abstract »    Full Text »    PDF »
Dominant negative mutation of the hematopoietic-specific Rho GTPase, Rac2, is associated with a human phagocyte immunodeficiency.
D. A. Williams, W. Tao, F. Yang, C. Kim, Y. Gu, P. Mansfield, J. E. Levine, B. Petryniak, C. W. Derrow, C. Harris, et al. (2000)
Blood 96, 1646-1654
   Abstract »    Full Text »    PDF »
Molecular analysis of 9 new families with chronic granulomatous disease caused by mutations in CYBA, the gene encoding p22phox.
J. Rae, D. Noack, P. G. Heyworth, B. A. Ellis, J. T. Curnutte, and A. R. Cross (2000)
Blood 96, 1106-1112
   Abstract »    Full Text »    PDF »
Processing and Maturation of Flavocytochrome b558 Include Incorporation of Heme as a Prerequisite for Heterodimer Assembly.
F. R. DeLeo, J. B. Burritt, L. Yu, A. J. Jesaitis, M. C. Dinauer, and W. M. Nauseef (2000)
J. Biol. Chem. 275, 13986-13993
   Abstract »    Full Text »    PDF »
Recombination events between the p47-phox gene and its highly homologous pseudogenes are the main cause of autosomal recessive chronic granulomatous disease.
J. Roesler, J. T. Curnutte, J. Rae, D. Barrett, P. Patino, S. J. Chanock, and A. Goerlach (2000)
Blood 95, 2150-2156
   Abstract »    Full Text »    PDF »
Polarization of Chemoattractant Receptor Signaling During Neutrophil Chemotaxis.
G. Servant, O. D. Weiner, P. Herzmark, T. Balla, J. W. Sedat, and H. R. Bourne (2000)
Science 287, 1037-1040
   Abstract »    Full Text »
Roles of PLC-2 and -3 and PI3K in Chemoattractant-Mediated Signal Transduction.
Z. Li, H. Jiang, W. Xie, Z. Zhang, A. V. Smrcka, and D. Wu (2000)
Science 287, 1046-1049
   Abstract »    Full Text »
Four novel mutations in the gene encoding gp91-phox of human NADPH oxidase: consequences for oxidase assembly.
J. H. W. Leusen, C. Meischl, M. H. M. Eppink, P. M. Hilarius, M. de Boer, R. S. Weening, A. Ahlin, L. Sanders, D. Goldblatt, H. Skopczynska, et al. (2000)
Blood 95, 666-673
   Abstract »    Full Text »    PDF »
Roles of phospholipid signaling in chemoattractant-induced responses.
D Wu, C. Huang, and H Jiang (2000)
J. Cell Sci. 113, 2935-2940
   Abstract »    PDF »
NADPH Oxidase Activation and Assembly During Phagocytosis.
F. R. DeLeo, L.-A. H. Allen, M. Apicella, and W. M. Nauseef (1999)
J. Immunol. 163, 6732-6740
   Abstract »    Full Text »    PDF »
Molecular Characterization of Autosomal Recessive Chronic Granulomatous Disease Caused by a Defect of the Nicotinamide Adenine Dinucleotide Phosphate (Reduced Form) Oxidase Component p67-phox.
P. J. Patino, J. Rae, D. Noack, R. Erickson, J. Ding, D. G. de Olarte, and J. T. Curnutte (1999)
Blood 94, 2505-2514
   Abstract »    Full Text »    PDF »
The p67phox Activation Domain Regulates Electron Flow from NADPH to Flavin in Flavocytochrome b558.
Y. Nisimoto, S. Motalebi, C.-H. Han, and J. D. Lambeth (1999)
J. Biol. Chem. 274, 22999-23005
   Abstract »    Full Text »    PDF »
Activation of Human Neutrophil NADPH Oxidase by Phosphatidic Acid or Diacylglycerol in a Cell-free System. ACTIVITY OF DIACYLGLYCEROL IS DEPENDENT ON ITS CONVERSION TO PHOSPHATIDIC ACID.
R. W. Erickson, P. Langel-Peveri, A. E. Traynor-Kaplan, P. G. Heyworth, and J. T. Curnutte (1999)
J. Biol. Chem. 274, 22243-22250
   Abstract »    Full Text »    PDF »
Essential Requirement of Cytosolic Phospholipase A2 for Activation of the H+ Channel in Phagocyte-like Cells.
A. Lowenthal and R. Levy (1999)
J. Biol. Chem. 274, 21603-21608
   Abstract »    Full Text »    PDF »
Stimulation of a Vascular Smooth Muscle Cell NAD(P)H Oxidase by Thrombin. EVIDENCE THAT p47phox MAY PARTICIPATE IN FORMING THIS OXIDASE IN VITRO AND IN VIVO.
C. Patterson, J. Ruef, N. R. Madamanchi, P. Barry-Lane, Z. Hu, C. Horaist, C. A. Ballinger, A. R. Brasier, C. Bode, and M. S. Runge (1999)
J. Biol. Chem. 274, 19814-19822
   Abstract »    Full Text »    PDF »
Receptor for Advanced Glycation End Products (RAGE)-mediated Neurite Outgrowth and Activation of NF-kappa B Require the Cytoplasmic Domain of the Receptor but Different Downstream Signaling Pathways.
H. J. Huttunen, C. Fages, and H. Rauvala (1999)
J. Biol. Chem. 274, 19919-19924
   Abstract »    Full Text »    PDF »
Phosphoinositide 3-Kinase-dependent and -independent Activation of the Small GTPase Rac2 in Human Neutrophils.
T. Akasaki, H. Koga, and H. Sumimoto (1999)
J. Biol. Chem. 274, 18055-18059
   Abstract »    Full Text »    PDF »
Activation of the Leukocyte NADPH Oxidase by Protein Kinase C in a Partially Recombinant Cell-free System.
L. R. Lopes, C. R. Hoyal, U. G. Knaus, and B. M. Babior (1999)
J. Biol. Chem. 274, 15533-15537
   Abstract »    Full Text »    PDF »
Transient Association of the Nicotinamide Adenine Dinucleotide Phosphate Oxidase Subunits p47phox and p67phox With Phagosomes in Neutrophils From Patients With X-Linked Chronic Granulomatous Disease.
L.-A. H. Allen, F. R. DeLeo, A. Gallois, S. Toyoshima, K. Suzuki, and W. M. Nauseef (1999)
Blood 93, 3521-3530
   Abstract »    Full Text »    PDF »
Characterization of Rac and Cdc42 Activation in Chemoattractant-stimulated Human Neutrophils Using a Novel Assay for Active GTPases.
V. Benard, B. P. Bohl, and G. M. Bokoch (1999)
J. Biol. Chem. 274, 13198-13204
   Abstract »    Full Text »    PDF »
Ca2+/Calmodulin-dependent Protein Kinase II Regulates Tiam1 by Reversible Protein Phosphorylation.
I. N. Fleming, C. M. Elliott, F. G. Buchanan, C. P. Downes, and J. H. Exton (1999)
J. Biol. Chem. 274, 12753-12758
   Abstract »    Full Text »    PDF »
Mutagenesis of an Arginine- and Lysine-rich Domain in the gp91phox Subunit of the Phagocyte NADPH-oxidase Flavocytochrome b558.
K. J. Biberstine-Kinkade, L. Yu, and M. C. Dinauer (1999)
J. Biol. Chem. 274, 10451-10457
   Abstract »    Full Text »    PDF »
Biosynthesis of Flavocytochrome b558. gp91phox IS SYNTHESIZED AS A 65-kDa PRECURSOR (p65) IN THE ENDOPLASMIC RETICULUM.
L. Yu, F. R. DeLeo, K. J. Biberstine-Kinkade, J. Renee, W. M. Nauseef, and M. C. Dinauer (1999)
J. Biol. Chem. 274, 4364-4369
   Abstract »    Full Text »    PDF »
Isolation and Characterization of a Variant HL60 Cell Line Defective in the Activation of the NADPH Oxidase by Phorbol Myristate Acetate.
M. Tardif, M.-J. Rabiet, T. Christophe, M.-D. Milcent, and F. Boulay (1998)
J. Immunol. 161, 6885-6895
   Abstract »    Full Text »    PDF »
Role of Histidines Identified by Mutagenesis in the NADPH Oxidase-associated H+ Channel.
L. M. Henderson (1998)
J. Biol. Chem. 273, 33216-33223
   Abstract »    Full Text »    PDF »
Prostaglandin F2{alpha} Treatment In Vivo, but Not In Vitro, Stimulates Protein Kinase C-Activated Superoxide Production by Nonsteroidogenic Cells of the Rat Corpus Luteum.
R. F. Aten, T. R. Kolodecik, M. J. Rossi, C. Debusscher, and H. R. Behrman (1998)
Biol Reprod 59, 1069-1076
   Abstract »    Full Text »
Effect of Cytotoxic Necrotizing Factor-1 on Actin Cytoskeleton in Human Monocytes: Role in the Regulation of Integrin-Dependent Phagocytosis.
C. Capo, S. Meconi, M.-V. Sanguedolce, N. Bardin, G. Flatau, P. Boquet, and J.-L. Mege (1998)
J. Immunol. 161, 4301-4308
   Abstract »    Full Text »    PDF »
Structural Requirements for PAK Activation by Rac GTPases.
U. G. Knaus, Y. Wang, A. M. Reilly, D. Warnock, and J. H. Jackson (1998)
J. Biol. Chem. 273, 21512-21518
   Abstract »    Full Text »    PDF »
Essential Requirement of Cytosolic Phospholipase A2 for Activation of the Phagocyte NADPH Oxidase.
R. Dana, T. L. Leto, H. L. Malech, and R. Levy (1998)
J. Biol. Chem. </