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Science 13 December 1991: Vol. 254. no. 5038, pp. 1636 - 1639 DOI: 10.1126/science.1749936
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Articles
Science, Vol 254, Issue 5038, 1636-1639
Copyright © 1991 by American Association for the Advancement of Science
Dopaminergic and ligand-independent activation of steroid hormone receptors
RF Power,
SK Mani,
J Codina,
OM Conneely,
and
BW O'Malley
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
The current view of how steroid hormone receptors affect gene transcription is that these receptors, on binding ligand, change to a state in which they can interact with chromatin and regulate transcription of target genes. Receptor activation is believed to be dependent only on this ligand-binding event. Selected steroid hormone receptors can be activated in a ligand-independent manner by a membrane receptor agonist, the neurotransmitter dopamine. In vitro, dopamine faithfully mimicked the effect of progesterone by causing a translocation of chicken progesterone receptor (cPR) from cytoplasm to nucleus. Dual activation by progesterone and dopamine was dissociable, and a serine residue in the cPR was identified that is not necessary for progesterone-dependent activation of cPR, but is essential for dopamine activation of this receptor.
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