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Science 20 December 1991:
Vol. 254. no. 5039, pp. 1808 - 1810
DOI: 10.1126/science.1722352
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Articles
Science, Vol 254, Issue 5039, 1808-1810
Copyright © 1991 by American Association for the Advancement of Science
Reverse transcriptase encoded by a human transposable element
SL Mathias,
AF Scott,
HH Kazazian Jr,
JD Boeke,
and
A Gabriel
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
L1 elements are highly repeated mammalian DNA sequences whose structure suggests dispersal by retrotransposition. A consensus L1 element encodes a protein with sequence similarity to known reverse transcriptases. The second open reading frame from the human L1 element L1.2A was expressed as a fusion protein targeted to Ty1 virus-like particles in Saccharomyces cerevisiae and shown to have reverse transcriptase activity. This activity was eliminated by a missense mutation in the highly conserved amino acid motif Y/F-X-D-D. Thus, L1 represents a potential source of the reverse transcriptase activity necessary for dispersion of the many classes of mammalian retroelements.
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