Related Content
Search Google Scholar for:
|
|
Science 3 July 1992:
Vol. 257. no. 5066, pp. 72 - 74
DOI: 10.1126/science.1621095
|
|
Articles
Science, Vol 257, Issue 5066, 72-74
Copyright © 1992 by American Association for the Advancement of Science
Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants
TA Voelker,
AC Worrell,
L Anderson,
J Bleibaum,
C Fan,
DJ Hawkins,
SE Radke,
and
HM Davies
Calgene, Inc., Davis, CA 95616.
Medium-chain fatty acids (FAs), found in storage lipids of certain plants, are an important renewable resource. Seeds of undomesticated California bay accumulate laurate (12:0), and a 12:0-acyl-carrier protein thioesterase (BTE) has been purified from this tissue. Sequencing of BTE enabled the cloning of a complementary DNA coding for a plastid-targeted preprotein. Expression of the complementary DNA in the seeds of Arabidopsis thaliana resulted in BTE activity, and medium chains accumulated at the expense of long-chain (greater than or equal to 16) FAs. Laurate became the most abundant FA species and was deposited in the storage triacylglycerols. These results demonstrate a mechanism for medium-chain FA synthesis in plants.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Modification of Brassica Oil Using Conventional and Transgenic Approaches.
- R. Scarth and J. Tang (2006)
Crop Sci.
46, 1225-1236
| Abstract »
| Full Text »
| PDF »
- A Structural Model of the Plant Acyl-Acyl Carrier Protein Thioesterase FatB Comprises Two Helix/4-Stranded Sheet Domains, the N-terminal Domain Containing Residues That Affect Specificity and the C-terminal Domain Containing Catalytic Residues.
- K. M. Mayer and J. Shanklin (2005)
J. Biol. Chem.
280, 3621-3627
| Abstract »
| Full Text »
| PDF »
- Production of Polyhydroxybutyrate by Polycistronic Expression of Bacterial Genes in Tobacco Plastid.
- Y. Arai, T. Shikanai, Y. Doi, S. Yoshida, I. Yamaguchi, and H. Nakashita (2004)
Plant Cell Physiol.
45, 1176-1184
| Abstract »
| Full Text »
| PDF »
- Stability of the Expression of Acyl-ACP Thioesterase Transgenes in Oilseed Rape Doubled Haploid Lines.
- J. Tang, R. Scarth, and P. B. E. McVetty (2004)
Crop Sci.
44, 732-740
| Abstract »
| Full Text »
| PDF »
- DGAT2 Is a New Diacylglycerol Acyltransferase Gene Family. PURIFICATION, CLONING, AND EXPRESSION IN INSECT CELLS OF TWO POLYPEPTIDES FROM MORTIERELLA RAMANNIANA WITH DIACYLGLYCEROL ACYLTRANSFERASE ACTIVITY.
- K. D. Lardizabal, J. T. Mai, N. W. Wagner, A. Wyrick, T. Voelker, and D. J. Hawkins (2001)
J. Biol. Chem.
276, 38862-38869
| Abstract »
| Full Text »
| PDF »
- Overexpression of 3-Ketoacyl-Acyl-Carrier Protein Synthase IIIs in Plants Reduces the Rate of Lipid Synthesis.
- K. Dehesh, H. Tai, P. Edwards, J. Byrne, and J. G. Jaworski (2001)
Plant Physiology
125, 1103-1114
| Abstract »
| Full Text »
- Accumulation of Palmitate in Arabidopsis Mediated by the Acyl-Acyl Carrier Protein Thioesterase FATB1.
- P. Dörmann, T. A. Voelker, and J. B. Ohlrogge (2000)
Plant Physiology
123, 637-644
| Abstract »
| Full Text »
- Accumulation of Very-Long-Chain Fatty Acids in Membrane Glycerolipids Is Associated with Dramatic Alterations in Plant Morphology.
- A. A. Millar, M. Wrischer, and L. Kunst (1998)
PLANT CELL
10, 1889-1902
| Abstract »
| Full Text »
| PDF »
- Expression of Lauroyl–Acyl Carrier Protein Thioesterase in Brassica napus Seeds Induces Pathways for Both Fatty Acid Oxidation and Biosynthesis and Implies a Set Point for Triacylglycerol Accumulation.
- V. S. Eccleston and J. B. Ohlrogge (1998)
PLANT CELL
10, 613-622
| Abstract »
| Full Text »
- Cloning, Expression in Yeast, and Functional Characterization of CYP81B1, a Plant Cytochrome P450 That Catalyzes In-chain Hydroxylation of Fatty Acids.
- F. Cabello-Hurtado, Y. Batard, J.-P. Salaun, F. Durst, F. Pinot, and D. Werck-Reichhart (1998)
J. Biol. Chem.
273, 7260-7267
| Abstract »
| Full Text »
| PDF »
- The Catalytic Cysteine and Histidine in the Plant Acyl-Acyl Carrier Protein Thioesterases.
- L. Yuan, B. A. Nelson, and G. Caryl (1996)
J. Biol. Chem.
271, 3417-3419
| Abstract »
| Full Text »
| PDF »
- Discovery of an Epidermal Stearoyl-Acyl Carrier Protein Thioesterase.
- D. Liu and D. Post-Beittenmiller (1995)
J. Biol. Chem.
270, 16962-16969
| Abstract »
| Full Text »
| PDF »
- Modification of Plant Lipid Synthesis.
- R. Topfer, N. Martini, and J. Schell (1995)
Science
268, 681-686
| Abstract »
| PDF »
- Engineered biosynthesis of novel polyketides.
- R McDaniel, S Ebert-Khosla, D. Hopwood, and C Khosla (1993)
Science
262, 1546-1550
| Abstract »
| PDF »
|
|
