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Science 24 July 1992:
Vol. 257. no. 5069, pp. 528 - 531
DOI: 10.1126/science.1636087
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Articles

Science, Vol 257, Issue 5069, 528-531
Copyright © 1992 by American Association for the Advancement of Science

articles

Recognition of angiotensin II: antibodies at different levels of an idiotypic network are superimposable

KC Garcia, SV Desiderio, PM Ronco, PJ Verroust, and LM Amzel

Department of Biophysics and Biophysical Chemistry, Johns Hopkins University Medical School, Baltimore, MD 21205.

Genetic and sequence information are reported for an angiotensin II-reactive antibody (Ab1, MAb 110) and an anti--anti-idiotypic antibody (Ab3, MAb 131) that have identical antigen binding properties and that are related by an anti-idiotypic antibody (Ab2-beta) that satisfies accepted biochemical criteria for an internal image-bearing antibody. The sequences of the variable regions of the Ab3 and of the Ab1 are nearly identical, even though the Ab1 is an antibody to a peptide and the Ab3 is an antibody to a globular protein. Significantly, amino acid residues that make critical contacts with antigen in the crystal structure of the Ab3-antigen complex are highly conserved in Ab1, suggesting that the epitopes of the Ab2-beta recognized by the Ab3 do indeed resemble the bound structure of the antigen.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Germline Structure and Differential Utilization of Igha and Ighb VH10 Genes.
E. A. Whitcomb, B. B. Haines, A. P. Parmelee, A. M. Pearlman, and P. H. Brodeur (1999)
J. Immunol. 162, 1541-1550
   Abstract »    Full Text »    PDF »
Characterization of anti-anti-idiotypic antibodies that bind antigen and an anti-idiotype.
F. A. Goldbaum, C. A. Velikovsky, W. Dall'Acqua, C. A. Fossati, B. A. Fields, B. C. Braden, R. J. Poljak, and R. A. Mariuzza (1997)
PNAS 94, 8697-8701
   Abstract »    Full Text »    PDF »
Three-dimensional structure of an angiotensin II-Fab complex at 3 A: hormone recognition by an anti-idiotypic antibody.
K. Garcia, P. Ronco, P. Verroust, A. Brunger, and L. Amzel (1992)
Science 257, 502-507
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)