Related Content
Search Google Scholar for:
|
|
Science 14 August 1992:
Vol. 257. no. 5072, pp. 958 - 961
DOI: 10.1126/science.1502560
|
|
Articles
Science, Vol 257, Issue 5072, 958-961
Copyright © 1992 by American Association for the Advancement of Science
Pseudo--half-knot formation with RNA
DJ Ecker,
TA Vickers,
TW Bruice,
SM Freier,
RD Jenison,
M Manoharan,
and
M Zounes
ISIS Pharmaceuticals, Carlsbad, CA 92008.
A pseudo--half-knot can be formed by binding an oligonucleotide asymmetrically to an RNA hairpin loop. This binding motif was used to target the human immunodeficiency virus TAR element, an important viral RNA structure that is the receptor for Tat, the major viral transactivator protein. Oligonucleotides complementary to different halves of the TAR structure bound with greater affinity than molecules designed to bind symmetrically around the hairpin. The pseudo--half-knot--forming oligonucleotides altered the TAR structure so that specific recognition and binding of a Tat-derived peptide was disrupted. This general binding motif may be used to disrupt the structure of regulatory RNA hairpins.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Liquid-crystal NMR structure of HIV TAR RNA bound to its SELEX RNA aptamer reveals the origins of the high stability of the complex.
- (2008)
PNAS
105, 9210-9215
- Synthesis, cellular uptake and HIV-1 Tat-dependent trans-activation inhibition activity of oligonucleotide analogues disulphide-conjugated to cell-penetrating peptides.
- J. J. Turner, A. A. Arzumanov, and M. J. Gait (2005)
Nucleic Acids Res.
33, 27-42
| Abstract »
| Full Text »
| PDF »
- Sensing complex regulatory networks by conformationally controlled hairpin ribozymes.
- S. H. Najafi-Shoushtari, G. Mayer, and M. Famulok (2004)
Nucleic Acids Res.
32, 3212-3219
| Abstract »
| Full Text »
| PDF »
- mRNA fusion constructs serve in a general cell-based assay to profile oligonucleotide activity.
- D. Husken, F. Asselbergs, B. Kinzel, F. Natt, J. Weiler, P. Martin, R. Haner, and J. Hall (2003)
Nucleic Acids Res.
31, e102
| Abstract »
| Full Text »
| PDF »
- Effects of RNA secondary structure on cellular antisense activity.
- T. A. Vickers, J. R. Wyatt, and S. M. Freier (2000)
Nucleic Acids Res.
28, 1340-1347
| Abstract »
| Full Text »
| PDF »
- DNA Aptamers Selected Against the HIV-1 trans-Activation-responsive RNA Element Form RNA-DNA Kissing Complexes.
- C. Boiziau, E. Dausse, L. Yurchenko, and J.-J. Toulme (1999)
J. Biol. Chem.
274, 12730-12737
| Abstract »
| Full Text »
| PDF »
- Resected RNA pseudoknots and their recognition by histidyl-tRNA synthetase.
- B. Felden and R. Giege (1998)
PNAS
95, 10431-10436
| Abstract »
| Full Text »
| PDF »
- PD-loop: A complex of duplex DNA with an oligonucleotide.
- N. O. Bukanov, V. V. Demidov, P. E. Nielsen, and M. D. Frank-Kamenetskii (1998)
PNAS
95, 5516-5520
| Abstract »
| Full Text »
| PDF »
- The Influence of Antisense Oligonucleotide-induced RNA Structure on Escherichia coli RNase H1 Activity.
- W. F. Lima, V. Mohan, and S. T. Crooke (1997)
J. Biol. Chem.
272, 18191-18199
| Abstract »
| Full Text »
| PDF »
- Combinatorial Screening and Rational Optimization for Hybridization to Folded Hepatitis C Virus RNA of Oligonucleotides with Biological Antisense Activity.
- W. F. Lima, V. Brown-Driver, M. Fox, R. Hanecak, and T. W. Bruice (1997)
J. Biol. Chem.
272, 626-638
| Abstract »
| Full Text »
| PDF »
|
|
