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Science 9 July 1993:
Vol. 261. no. 5118, pp. 206 - 208
DOI: 10.1126/science.8392224
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Articles
Science, Vol 261, Issue 5118, 206-208
Copyright © 1993 by American Association for the Advancement of Science
An NAD derivative produced during transfer RNA splicing: ADP-ribose 1"-2" cyclic phosphate
GM Culver,
SM McCraith,
M Zillmann,
R Kierzek,
N Michaud,
RD LaReau,
DH Turner,
and
EM Phizicky
Department of Biochemistry, University of Rochester School of Medicine and Dentistry, NY 14642.
Transfer RNA (tRNA) splicing is essential in Saccharomyces cerevisiae as well as in humans, and many of its features are the same in both. In yeast, the final step of this process is removal of the 2' phosphate generated at the splice junction during ligation. A nicotinamide adenine dinucleotide (NAD)-dependent phosphotransferase catalyzes removal of the 2' phosphate and produces a small molecule. It is shown here that this small molecule is an NAD derivative: adenosine diphosphate (ADP)-ribose 1"-2" cyclic phosphate. Evidence is also presented that this molecule is produced in Xenopus laevis oocytes as a result of dephosphorylation of ligated tRNA.
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