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Articles
Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip
1 Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2
Micromachining technology was used to prepare chemical analysis systems on glass chips (1 centimeter by 2 centimeters or larger) that utilize electroosmotic pumping to drive fluid flow and electrophoretic separation to distinguish sample components. Capillaries 1 to 10 centimeters long etched in the glass (cross section, 10 micrometers by 30 micrometers) allow for capillary electrophoresis-based separations of amino acids with up to 75,000 theoretical plates in about 15 seconds, and separations of about 600 plates can be effected within 4 seconds. Sample treatment steps within a manifold of intersecting capillaries were demonstrated for a simple sample dilution process. Manipulation of the applied voltages controlled the directions of fluid flow within the manifold. The principles demonstrated in this study can be used to develop a miniaturized system for sample handling and separation with no moving parts. Submitted on March 10, 1993Accepted on June 8, 1993
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Science. ISSN 0036-8075 (print), 1095-9203 (online)
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