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Science 8 July 1994:
Vol. 265. no. 5169, pp. 248 - 252
DOI: 10.1126/science.8023143
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Articles

Science, Vol 265, Issue 5169, 248-252
Copyright © 1994 by American Association for the Advancement of Science

articles

Lack of evidence for the dichotomy of TH1 and TH2 predominance in HIV-infected individuals

C Graziosi, G Pantaleo, KR Gantt, JP Fortin, JF Demarest, OJ Cohen, RP Sekaly, and AS Fauci

Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

A switch from a T helper 1 (TH1) cytokine phenotype to a TH2 phenotype has been proposed as a critical element in the progression of human immunodeficiency virus (HIV) disease. Here, constitutive cytokine expression was analyzed in unfractionated and sorted cell populations isolated from peripheral blood and lymph nodes of HIV-infected individuals at different stages of disease. Expression of interleukin-2 (IL-2) and IL-4 was barely detectable (or undetectable) regardless of the stage of disease. CD8+ cells expressed large amounts of interferon gamma and IL-10, and the levels of these cytokines remained stably high throughout the course of infection. Furthermore, similar patterns of cytokine expression were observed after stimulation in vitro of purified CD4+ T cell populations obtained from HIV-infected individuals at different stages of disease. These results indicate that a switch from the TH1 to the TH2 cytokine phenotype does not occur during the progression of HIV disease.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)