Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 5 August 1994:
Vol. 265. no. 5173, pp. 762 - 765
DOI: 10.1126/science.265.5173.762
Prev | Table of Contents | Next

Articles

Antibody-Mediated Bacteriorhodopsin Orientation for Molecular Device Architectures

Koichi Koyama 1, Naoto Yamaguchi 1, and Tsutomu Miyasaka 1

1 Ashigara Research Laboratories, Fuji Photo Film Co. Ltd., Minamiashigara, Kanagawa, 250-01, Japan

A rational method for constructing highly oriented films of purple membrane (PM) has been established by using two kinds of bispecific antibodies with different antigen-binding sites, one binding to a specific side of bacteriorhodopsin and the other to a phospholipid hapten. A hapten monolayer deposited on a metal electrode was treated with a bispecific antibody solution and incubated with a PM suspension to produce a highly oriented PM film, as confirmed by electron microscopy in which an immunogold labeling technique was used. This antibody-mediated PM monolayer was then used in the construction of a light-sensing photoelectric device. A comparison of the two incorporated PM monolayers showed that highly efficient photocurrents were produced by the PM monolayer whose unidirectionally oriented cytoplasmic surface faces the electrode.

Submitted on March 18, 1994
Accepted on May 31, 1994


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
AFM and TEM observations of {alpha}-helix to {beta}-sheet conformational change occurring on carbon nanotubes.
Y. Sugiyama, Y. Inoue, E. Muneyuki, H. Haneda, and M. Fujimoto (2006)
J. Electron Microsc. (Tokyo) 55, 143-149
   Abstract »    Full Text »    PDF »
Approaches for biological and biomimetic energy conversion.
D. A. LaVan and J. N. Cha (2006)
PNAS 103, 5251-5255
   Abstract »    Full Text »    PDF »
High-Performance Photovoltaic Behavior of Oriented Purple Membrane Polymer Composite Films.
L. Zhang, T. Zeng, K. Cooper, and R. O. Claus (2003)
Biophys. J. 84, 2502-2507
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)